We currently use the Promega "DeadEnd Colorimetric TUNEL System" kit
with excellent results. I have also programmed it on my Ventana
Discovery instrument; so it is completely automated. We follow the
protocol included in the kit and the only reagent not included is the
DNase which we use at a 1:20 dilution for 30 minutes at 37 degrees C. We
get our "DNA POLIDNase 1" from Invitogen cat # 18162016. Instead of the
4% paraformaldehyde, we use 37% formaldehyde to make a 10% solution in
PBS. Hope this helps.

Charlene Henry, HT QIHC
Histology/IHC Section Head
Department of Pathology
St. Jude Children's Research Hospital
332 North Lauderdale
Memphis, TN  38105
Phone: 901-495-3349
FAX: 901-495-3100


-----Original Message-----
From: Patti Loykasek [mailto:ploykasek@phenopath.com] 
Sent: Wednesday, May 19, 2004 11:19 AM
To: histonet
Subject: [Histonet] Tunel

I'm looking for some tips, info on tunel assays, specifically the kit
from
Promega - "dead end" tunel kit. Thanks for the help.

Patti Loykasek


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