RE: [Histonet] Transportation of frozen sections

From:"Serrano del Pozo, Erika"

Hi Jorge Ivan,

If you want to perform some immunohistochemistry or tunnel assay, sections
must keep frozen, so you should make a shippment to Colombia with dry ice. 

If they defrost, proteins will degradate and it woudn┤t be able to perform a
good antigen detection (IHC). No idea about ordinary stainings... depending
what you want to detect, I think better not defrost.


Erika Serrano
Research Assistant
Centre de Regulaciˇ Gen˛mica (CRG)
Barcelona -SPAIN- 





> ----------
> From: 	Jorge Ivan Zapata Valencia
> Sent: 	Saturday, May 15, 2004 3:33 AM
> To: 	histonet@lists.utsouthwestern.edu
> Subject: 	[Histonet] Transportation of frozen sections
> 
> Hello everybody
> I want to ask how can I transport frozen sections between two distant 
> places? I am now in Japan but I have to go back to Colombia soon and
> finish 
> there the staining protocols I have to do. How I can transport the slides
> in 
> such a way that my samples arrive to Colombia in good condition to be 
> stained? Must i fix them and keep them at room temperature?
> Any suggestion is really appreciated
> Thank You very much
> Regards,
> 
> JORGE IVAN ZAPATA
> 
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