RE: [Histonet] Testes Bouin's fluid
These tissues are small enough that they don't need to be fixed for 24
hours. Try 8 to 12 hours, skip the NBF, wash in water for a bit, and rinse
with several changes of 70% ETOH to remove picric acid. More acid will
leach out during processing. Provided the tissues are no more than 3mm
thick, 2-3 hours in 80% ETOH and 1 hour each for the rest of your
processing should give you decent sections that will stain adequately with
any H&E. If you don't leach out enough picric acid, your sections will
compress when cutting, so use ice.
Just another way to do it.
Mary Lou Norman
College of Veterinary Medicine
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