RE: [Histonet] Formalin fixation of cells for paraffin embedding?
Joanne,
Would one of the antigens that you speak of that doesn't like alcohol
fixation be Thyroglobulin?
Marsha Linville
DCL Medical Laboratory
Indianapolis, IN 46268
(317) 874-1232
-----Original Message-----
From: Joanne Mauger [mailto:MAUGER@email.chop.edu]
Sent: Friday, May 21, 2004 6:52 AM
To: eca9@georgetown.edu; histonet@lists.utsouthwestern.edu
Cc: histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] Formalin fixation of cells for paraffin
embedding?
To Eva,
Wrap the pellet in lens paper,and put it in a cassette to fix and process
with your tissues. I would skip the alcohol fixation and spin the cells down
in NBF, then proceed with processing and embed as usual. Some antigens don't
like alcohol fix.
Jo
>>> "Eva C. Andersson" 05/20/04 03:18PM >>>
Hi everyone,
My lab wants to paraffin embed some cells to be used as positive
controls for immunohisochemistry staining.
We have a cell line growing but need a good protocol for fixing the
cells with formalin. Once the cells have been fixed the pellet will be
embedded in paraffin by our histopathology department.
Does anyone have a good and easy method for the fixation part?
We will deliver the pellet to the histopathology department in 70% ethanol.
Thanks in advance,
Eva Andersson
Research Assistant
Georgetown University
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
<< Previous Message | Next Message >>