I have never tried the pure isomers myself, but three of my professors told
me that all 3 or any mixture thereof were equally effective. (They probably
just heard it from their professors.) The mixed xylenes (which also contain
some ethylbenzene) are a little cheaper than m-xylene and much cheaper than
the other pure isomers.

Allen A. Smith, Ph.D.
Professor of Anatomy
Barry University
School of Graduate Medical Sciences
            Podiatric Medicine and Surgery
Miami Shores, Florida


-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Tony Henwood
Sent: Tuesday, May 18, 2004 9:09 PM
To: Histonet (Histonet@lists.utsouthwestern.edu);
'histonet@pathology.swmed.edu'
Subject: [Histonet] Efficiency of p o and m xylenes in dewaxing

Hi all,

Would anyone have any information on the relative efficiencies of para, meta
and ortho xylenes in dewaxing paraffin sections for nucleic acid extraction.

Does it matter?

Regards

Tony Henwood JP, BAppSc, GradDipSysAnalys, CT(ASC)
Laboratory Manager
The Children's Hospital at Westmead,
Locked Bag 4001, Westmead, 2145, AUSTRALIA.
Tel: 612 9845 3306
Fax: 612 9845 3318




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