RE: [Histonet] CJD DECONTAMINATION PROCEDURE
Your probably going to have to process by hand. Formic acid for one
hour, the formalin overnight. Then just routinely process by hand.
Everything in disposable containers.
Use 5N sodium hydroxide to all of the processing solutions to
"neutrilize" and have your bioharzard people come in a get it. Wet
down everything with 5N sodium hydroxide and let it sit for a bit.
Then wipe it off with wet paper towels. Everything should be red
bagged and incinerated. I used to work for the Brain Bank for AD
We used to have fits about Dr's performing biopsy's on patients
suspect of CJD, because CJD doesn't show up everywhere in the brain
and the chances that they are going to get the peice that shows
evidence of CJ is slim. And I don't know of any treatment for CJD so,
the point of the biopsy? Hopefully the surgical team will be using
disposables and wetting everything in the OR down with 5N sodium
hydroxide before continueing to the next patient.
You should also store the blocks and slides seperate from everything
else. We had a speical area devoted to such cases.
Any other questions feel free to ask.
Hope that helps a little. We didn't get any hazard pay!!
Loralee Gehan, HTL
>From: "Connie McManus"
>Subject: RE: [Histonet] CJD DECONTAMINATION PROCEDURE
>Date: Wed, 19 May 2004 08:03:16 -0600
>I don't deal with CJD, but I do a lot of CWD (Chronic Wasting
>and Scrapie, which are prion diseases in dear/elk and sheep
>(respectively). CWD and Scrapie had been associated with humans for
>hundreds of years with no known transmission of the disease from
>to human. HOWEVER, since prions are not neutralized by fixation of
>kind, not killed by autoclaving, this makes them a biohazard in
>If I worked with CJD, I would ...
>1 set up a place that is completely separate from the rest of the
>Barrier off a section of your lab, use another room (preferable)
>2 use disposable bunny suits, shoe covers, face masks, eye wear and
>3 put stickie mats on the floor completely surrounding the cutting
>4 put ALL paraffin shavings in a biohaz bag,
>5 use paper towels to clean off the microtome and throw those in the
>6. incinerate the bunny suit, goggles, gloves, face maskes, etc with
>7. Ask for a really big pay raise. Claim it's hazard pay.
>Utah Veterinary Diagnostics Laboratory
>Utah State University
>[mailto:firstname.lastname@example.org] On Behalf Of
>Sent: Tuesday, May 18, 2004 9:59 AM
>Subject: [Histonet] CJD DECONTAMINATION PROCEDURE
>This question might have been posted before, but, Histology
>expecting a brain biopsy from a possible CJD patient today. The
>will be coming down in formalin from the O.R. It will be kept in
>for 2-7 days and will be transferred to formic acid for the required
>Then the specimen will be transferred to formalin before processing
>another 2 days. Has anyone dealt with this procedure before. These
>questions asked by the hstology department.
>How do you decontaminate the processor, microtome, stainer and
>What kind of protective gear apart from standard precaution has to be
>How about the shaving of the tissues?
>How do you dispose the formalin from the original formalin container
>the specimen was sent in and how do you dispose the reagents from the
>processor and the stainer?
>Can this biopsy be processed, once treated with formic acid, along
>other specimens or should the biopsy be processed with the other
>Thank you in advance
>Holy Name Hospital
>Teaneck, NJ 07666
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