RE: [Histonet] CJD DECONTAMINATION PROCEDURE
I don't deal with CJD, but I do a lot of CWD (Chronic Wasting Disease)
and Scrapie, which are prion diseases in dear/elk and sheep
(respectively). CWD and Scrapie had been associated with humans for
hundreds of years with no known transmission of the disease from animal
to human. HOWEVER, since prions are not neutralized by fixation of any
kind, not killed by autoclaving, this makes them a biohazard in
If I worked with CJD, I would ...
1 set up a place that is completely separate from the rest of the lab.
Barrier off a section of your lab, use another room (preferable)
2 use disposable bunny suits, shoe covers, face masks, eye wear and
3 put stickie mats on the floor completely surrounding the cutting
4 put ALL paraffin shavings in a biohaz bag,
5 use paper towels to clean off the microtome and throw those in the
6. incinerate the bunny suit, goggles, gloves, face maskes, etc with the
7. Ask for a really big pay raise. Claim it's hazard pay.
Utah Veterinary Diagnostics Laboratory
Utah State University
[mailto:firstname.lastname@example.org] On Behalf Of
Sent: Tuesday, May 18, 2004 9:59 AM
Subject: [Histonet] CJD DECONTAMINATION PROCEDURE
This question might have been posted before, but, Histology Department
expecting a brain biopsy from a possible CJD patient today. The
will be coming down in formalin from the O.R. It will be kept in
for 2-7 days and will be transferred to formic acid for the required
Then the specimen will be transferred to formalin before processing for
another 2 days. Has anyone dealt with this procedure before. These are
questions asked by the hstology department.
How do you decontaminate the processor, microtome, stainer and
What kind of protective gear apart from standard precaution has to be
How about the shaving of the tissues?
How do you dispose the formalin from the original formalin container
the specimen was sent in and how do you dispose the reagents from the
processor and the stainer?
Can this biopsy be processed, once treated with formic acid, along with
other specimens or should the biopsy be processed with the other
Thank you in advance
Holy Name Hospital
Teaneck, NJ 07666
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