From:"Bartlett, Jeanine"

The WHO booklet was too large to send as an attachment so it couldn't be
delivered.  I will try and send you what I think is the most relevant
chapter.  Perhaps you could visit the WHO site and get more information
if needed.  In the meantime, the Surveillance Center link below  should
have enough information.



There is a lot of conflicting information out there but here is
information from 2 very credible sources for you to review. One is the
National Surveillance Center at case Western and the other is the WHO.

Jeanine Bartlett, HT(ASCP)
Centers for Disease Control
Infectious Disease Pathology Activity
1600 Clifton Road, MS/G-32
Atlanta, GA 30333 and the WHO booklet is sent as an

-----Original Message-----
[] On Behalf Of
Sent: Tuesday, May 18, 2004 12:59 PM

Hello everyone,

This question might have been posted before, but,   Histology Department
expecting a brain biopsy from a possible CJD patient today.  The
specimen will be coming down in formalin from the O.R.  It will be kept
in formalin for 2-7 days and will be transferred to formic acid for the
required time. Then the specimen will be transferred to formalin before
processing for another 2 days.  Has anyone dealt with this procedure
before. These are the questions asked by the hstology department.

How do you decontaminate the processor, microtome, stainer and
coverslipper? What kind of protective gear apart from standard
precaution has to be used? How about the shaving of the tissues? How do
you dispose the formalin  from the original formalin container where the
specimen was sent in and how do you dispose the reagents from the
processor and the stainer? Can this biopsy be processed, once treated
with formic acid, along with the other specimens or  should the biopsy
be processed with the other specimens?

Need Help!!

Thank you in advance

Mala Srishan
Histology Supervisor
Holy Name Hospital
Teaneck, NJ 07666

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