[Histonet] where's the eosin?

From:"Gudrun Lang"

Dear netters
We process the prostatatissue in makro-cassettes in double time of the normal schedule in a VIP. NBF-70-80-96-100-Shell.sol-Paraffin total for 24 hours. The large sections are stained H&E by hand. (self-made Mayer's haematox. 20 min; selfmade 2% Eosin with a few drops of glacial acetic acid 8 min ).
Recently it happend for two times, that the tissue doesn't accept the Eosin. The sections remained blue and had only a shade of red. Normally our HE's are almost purple. Funny is, that in one case, the first slides were stained a few days before and looked good. The following slides of the same blocks remained blue without red.
Could anyone give me a hint? Thanks in advance.
Gudrun Lang

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