[Histonet] help

From:"Williams, Blanche"

Can someone  answer a question regarding slide retention for me? How long to
labs keep their immunofluorescence slides?

-----Original Message-----
From: histonet-request@lists.utsouthwestern.edu
[mailto:histonet-request@lists.utsouthwestern.edu]
Sent: Wednesday, May 19, 2004 10:16 AM
To: histonet@lists.utsouthwestern.edu
Subject: Histonet Digest, Vol 6, Issue 28


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Today's Topics:

   1. CJD DECONTAMINATION PROCEDURE (srishan@mail.holyname.org)
   2. RE: H&E stain problems (Gary Gill)
   3. RE: CJD DECONTAMINATION PROCEDURE (Bartlett, Jeanine)
   4. RE: CJD DECONTAMINATION PROCEDURE (Kari Bradshaw)
   5. research charges (Diane M Nelson)
   6. RE: CJD DECONTAMINATION PROCEDURE (Monson, Frederick )
   7. Presto Fix - ? (Richard Cartun)
   8. Re: Aussies and Kiwis standing up at microtomes (P. Emry)
   9. Congo Red (Karen Wittler)
  10. Re: MIcrotome alignment (Ford Royer)
  11. RE: research charges (Mass Histology Service)
  12. Efficiency of p o and m xylenes in dewaxing (Tony Henwood)
  13. Efficiency of p o and m xylenes in dewaxing (Tony Henwood)
  14. HTL tissue size minimum requriements  (Tara Mciver)
  15. unsubscribe (Weems, Joyce)
  16. (no subject) (yan gao)
  17. (no subject) (yan gao)
  18. (no subject) (yan gao)
  19. RE: HTL tissue size minimum requriements  (Galbraith, Joe)
  20. (no subject) (phyllis)
  21. RE: H&E stain problems (Connie McManus)
  22. RE: H&E stain problems
      (Marshall Terry Dr,	Consultant Histopathologist)
  23. RE: CJD DECONTAMINATION PROCEDURE (Connie McManus)


----------------------------------------------------------------------

Message: 1
Date: Tue, 18 May 2004 12:58:33 -0400
From: srishan@mail.holyname.org
Subject: [Histonet] CJD DECONTAMINATION PROCEDURE
To: histonet@lists.utsouthwestern.edu
Message-ID:
	

	
Content-Type: text/plain; charset=US-ASCII





Hello everyone,

This question might have been posted before, but,   Histology Department is
expecting a brain biopsy from a possible CJD patient today.  The specimen
will be coming down in formalin from the O.R.  It will be kept in formalin
for 2-7 days and will be transferred to formic acid for the required time.
Then the specimen will be transferred to formalin before processing for
another 2 days.  Has anyone dealt with this procedure before. These are the
questions asked by the hstology department.

How do you decontaminate the processor, microtome, stainer and
coverslipper?
What kind of protective gear apart from standard precaution has to be used?
How about the shaving of the tissues?
How do you dispose the formalin  from the original formalin container where
the specimen was sent in and how do you dispose the reagents from the
processor and the stainer?
Can this biopsy be processed, once treated with formic acid, along with the
other specimens or  should the biopsy be processed with the other
specimens?

Need Help!!

Thank you in advance

Mala Srishan
Histology Supervisor
Holy Name Hospital
Teaneck, NJ 07666




------------------------------

Message: 2
Date: Tue, 18 May 2004 12:03:43 -0500
From: Gary Gill 
Subject: RE: [Histonet] H&E stain problems
To: 'Connie McManus' , 	"'Marshall Terry
	Dr,Consultant Histopathologist'"
,
	'Petia P Stefanova'	,
'Megan
	Kear'	,
	histonet@lists.utsouthwestern.edu
Cc: histonet@lists.utsouthwestern.edu
Message-ID:
	
Content-Type: text/plain

You may have heard that classical music was composed by dead white
Europeans.  Well, Harris hematoxylin was composed by a dead white American
(physician at Jefferson Hospital in 1904).  Gill hematoxylin was composed by
a live white American.  So if you want to liven things up, go with Gill's!

Gary Gill (one and the same)

PS -- No royalties involved, thanks to bad advice in 1972 from corporate
counsel for Johns Hopkins Medical School.

-----Original Message-----
From: Connie McManus [mailto:convmcm@cc.usu.edu] 
Sent: Tuesday, May 18, 2004 10:41 AM
To: 'Marshall Terry Dr,Consultant Histopathologist'; 'Petia P Stefanova';
'Megan Kear'; histonet@lists.utsouthwestern.edu
Cc: histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] H&E stain problems


Wow.  What a lot of interesting comments!!  

I agree with Terry re the agitation.  When I watch the stainer do those dips
(I can program how many, but NOT the briskness), I wonder if you could even
call it agitation.  My hand dips are very brisk.  Also, I don't bother
letting the slides stay in the alcohols for 1 minute or 2, I give the slides
about 20 -30 good brisk dips in each solution, then the timed rinses &
staining.  This has always been far more satisfactory to me than those
sllllooooowwwww dips from the machine.

As for the kind of hematoxylin, someone suggested I throw out the Harris and
do Gills III.  I've tried Gill III before and I much prefer the Harris.  So
it's just a matter of personal preference on that... AND what your
pathologist likes *G*

Everyone having an nice Tuesday??? *g*

Connie McManus
Utah Veterinary Diagnostics Laboratory
Utah State University
Logan, UT
Phone:  435/797-1891
fax: 435/797-2805


-----Original Message-----
From: Marshall Terry Dr, Consultant Histopathologist
[mailto:Terry.Marshall@rothgen.nhs.uk] 
Sent: Tuesday, May 18, 2004 7:35 AM
To: Connie McManus; Petia P Stefanova; Megan Kear;
histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] H&E stain problems

Connie remarks:

"In truth, I prefer my hand stained sections better than when they're
stained automatically."

When I first saw what I call x-y stainers, I thought that we had in this,
something that reproduced hand staining. Not so. What I think is lacking is
the brisk agitation necessary to break down the interface between old and
new solution. I'm not so sure about the rinsing either.

Dr Terry L Marshall, B.A.(Law), M.B.,Ch.B.,F.R.C.Path  Consultant
Pathologist  Rotherham General Hospital  South Yorkshire  England
        terry.marshall@rothgen.nhs.uk

-----Original Message-----
From: Connie McManus [mailto:convmcm@cc.usu.edu]
Sent: 18 May 2004 15:09
To: 'Petia P Stefanova'; 'Megan Kear'; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] H&E stain problems


We use almost the exact same protocol... we use Surgipath Harris, but we
prepare eosin in-house.  One thing I am amazed at in this protocol is the
length of time in the acid alcohol.  Do you use an autostainer?  We have a
Leica.  The time is set to 1 second in the acid ETOH and
sometimes the sections are almost too differentiated.   I can't imagine
6 seconds in the acid ETOH!!  Even when I do H&E manually, I dip the slides
in and quickly put them in running water.  I must have a very strong
solution, I guess. Hmmmm. Interesting.  In truth, I prefer my hand stained
sections better than when they're stained automatically.

Just wondering and blabbering (hey, it's Tuesday, what do you expect??)

Connie McManus
Utah Veterinary Diagnostics Laboratory
Utah State University
Logan, UT
Phone:  435/797-1891
fax: 435/797-2805


-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Petia P
Stefanova
Sent: Monday, May 17, 2004 6:45 AM
To: Megan Kear; histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] H&E stain problems

Hi,

I use Harris's hematoxylin which is also regressive and purchase my
hematoxylin and eosin /alcohol-based/ from www.surgipath.com. I get very
good H&E staining with this protocol.

REAGENT                       TIME
Xylene                                3 min.
Xylene                                3 min.
Abs. alc.                              2 min.
Abs. alc.                              2 min
95% alc.                              2 min
80% alc.                              2 min
Wash /tap water/                  30 sec.
Hematoxylin                          8 min.
Wash /tap water/                  2 min.
Acid alcohol                          6 sec.
Wash /tap water/                  2 min.
Wash /tap water/                  5 min
80% alc.                              30 sec.
Eosin                                    15 sec.
95% alc.                              10 sec.
Abs. alc.                              30 sec.
Abs. alc.                              30 sec.
Xylene                                1 min.
Xylene                                1 min.
Xylene                                Exit

Hope it helps!
Petia


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http://lists.utsouthwestern.edu/mailman/listinfo/histonet


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http://lists.utsouthwestern.edu/mailman/listinfo/histonet


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------------------------------

Message: 3
Date: Tue, 18 May 2004 13:58:07 -0400
From: "Bartlett, Jeanine" 
Subject: RE: [Histonet] CJD DECONTAMINATION PROCEDURE
To: ,	
Message-ID:
	
Content-Type: text/plain;	charset="us-ascii"

The WHO booklet was too large to send as an attachment so it couldn't be
delivered.  I will try and send you what I think is the most relevant
chapter.  Perhaps you could visit the WHO site and get more information
if needed.  In the meantime, the Surveillance Center link below  should
have enough information.


Jeanine

-
Mala:

There is a lot of conflicting information out there but here is
information from 2 very credible sources for you to review. One is the
National Surveillance Center at case Western and the other is the WHO.

Jeanine Bartlett, HT(ASCP)
Centers for Disease Control
Infectious Disease Pathology Activity
1600 Clifton Road, MS/G-32
Atlanta, GA 30333

http://www.cjdsurveillance.com and the WHO booklet is sent as an
attachment.



-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of
srishan@mail.holyname.org
Sent: Tuesday, May 18, 2004 12:59 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] CJD DECONTAMINATION PROCEDURE






Hello everyone,

This question might have been posted before, but,   Histology Department
is
expecting a brain biopsy from a possible CJD patient today.  The
specimen will be coming down in formalin from the O.R.  It will be kept
in formalin for 2-7 days and will be transferred to formic acid for the
required time. Then the specimen will be transferred to formalin before
processing for another 2 days.  Has anyone dealt with this procedure
before. These are the questions asked by the hstology department.

How do you decontaminate the processor, microtome, stainer and
coverslipper? What kind of protective gear apart from standard
precaution has to be used? How about the shaving of the tissues? How do
you dispose the formalin  from the original formalin container where the
specimen was sent in and how do you dispose the reagents from the
processor and the stainer? Can this biopsy be processed, once treated
with formic acid, along with the other specimens or  should the biopsy
be processed with the other specimens?

Need Help!!

Thank you in advance

Mala Srishan
Histology Supervisor
Holy Name Hospital
Teaneck, NJ 07666


_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet



------------------------------

Message: 4
Date: Tue, 18 May 2004 10:55:17 -0700
From: "Kari Bradshaw" 
Subject: RE: [Histonet] CJD DECONTAMINATION PROCEDURE
To: , 
Message-ID: 
Content-Type: text/plain;	charset="US-ASCII"

These people have all the answers:
National Prion Pathology Surveillance Center
Case Western Reserve University
2085 Adelbert Road
Cleveland,Ohio  44106
216-368-0587
www.cjdsurveillance.com

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of
srishan@mail.holyname.org
Sent: Tuesday, May 18, 2004 9:59 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] CJD DECONTAMINATION PROCEDURE






Hello everyone,

This question might have been posted before, but,   Histology Department is
expecting a brain biopsy from a possible CJD patient today.  The specimen
will be coming down in formalin from the O.R.  It will be kept in formalin
for 2-7 days and will be transferred to formic acid for the required time.
Then the specimen will be transferred to formalin before processing for
another 2 days.  Has anyone dealt with this procedure before. These are the
questions asked by the hstology department.

How do you decontaminate the processor, microtome, stainer and
coverslipper?
What kind of protective gear apart from standard precaution has to be used?
How about the shaving of the tissues?
How do you dispose the formalin  from the original formalin container where
the specimen was sent in and how do you dispose the reagents from the
processor and the stainer?
Can this biopsy be processed, once treated with formic acid, along with the
other specimens or  should the biopsy be processed with the other
specimens?

Need Help!!

Thank you in advance

Mala Srishan
Histology Supervisor
Holy Name Hospital
Teaneck, NJ 07666


_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet



------------------------------

Message: 5
Date: Tue, 18 May 2004 13:03:49 -0500
From: Diane M Nelson 
Subject: [Histonet] research charges
To: histonet@lists.utsouthwestern.edu
Message-ID:
	<6.1.0.6.2.20040518124918.01b5caa0@dmnelson.mail.iastate.edu>
Content-Type: text/plain; charset="us-ascii"; format=flowed

Hi Histonetters!
Our veterinary lab is in the process of up dating our research charges. I 
would like to know what other labs/hospitals charge for H & E bone slides 
and H & E eye slides. Currently we are charging, Bone: $19.00 for 
decalcifying in 25% formic acid, process, embed, sectioning and H & E 
stain. We are losing money on our larger bone projects. Our charges for 
processing, embedding sectioning and H & E stain for eye slides is $9.00. 
It also depends on the project rather or not we make or lose money. The 
researchers do their own grossing of tissue. Any help would be greatly 
appreciated! Thanks in advance.

Diane Gerjets
Iowa State University




------------------------------

Message: 6
Date: Tue, 18 May 2004 14:04:45 -0400
From: "Monson, Frederick " 
Subject: RE: [Histonet] CJD DECONTAMINATION PROCEDURE
To: 
Cc: histonet@lists.utsouthwestern.edu
Message-ID:
	
Content-Type: text/plain;	charset="us-ascii"

http://www.nursingceu.com/NCEU/courses/prions/

http://www.mad-cow.org/tonsil_test2.html#Biosafety

A start.

Cheers

 Frederick C. Monson, PhD
Center for Advanced Scientific Imaging
Mail to Geology
West Chester University of Pennsylvania
Schmucker II Science Center, Room SS024
South Church Street and Rosedale Avenue
West Chester, PA, 19383
Phone/FAX:  610-738-0437
eMail:  fmonson@wcupa.edu
CASI Page and Scheduling
        http://darwin.wcupa.edu/CASI/


-----Original Message-----
From: srishan@mail.holyname.org [mailto:srishan@mail.holyname.org]
Sent: Tuesday, May 18, 2004 12:59 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] CJD DECONTAMINATION PROCEDURE






Hello everyone,

This question might have been posted before, but,   Histology Department
is
expecting a brain biopsy from a possible CJD patient today.  The
specimen will be coming down in formalin from the O.R.  It will be kept
in formalin for 2-7 days and will be transferred to formic acid for the
required time. Then the specimen will be transferred to formalin before
processing for another 2 days.  Has anyone dealt with this procedure
before. These are the questions asked by the hstology department.

How do you decontaminate the processor, microtome, stainer and
coverslipper? What kind of protective gear apart from standard
precaution has to be used? How about the shaving of the tissues? How do
you dispose the formalin  from the original formalin container where the
specimen was sent in and how do you dispose the reagents from the
processor and the stainer? Can this biopsy be processed, once treated
with formic acid, along with the other specimens or  should the biopsy
be processed with the other specimens?

Need Help!!

Thank you in advance

Mala Srishan
Histology Supervisor
Holy Name Hospital
Teaneck, NJ 07666


_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet



------------------------------

Message: 7
Date: Tue, 18 May 2004 14:32:48 -0400
From: "Richard Cartun" 
Subject: [Histonet] Presto Fix - ?
To: 
Message-ID: 
Content-Type: text/plain; charset=US-ASCII

Anyone using "Presto Fix" for bone marrow core biopsies?  If so, what
does it do to immunoreactivity?  Thank you.

Richard Cartun



------------------------------

Message: 8
Date: Tue, 18 May 2004 11:34:18 -0700 (PDT)
From: "P. Emry" 
Subject: Re: [Histonet] Aussies and Kiwis standing up at microtomes
To: Andrew Kennedy 
Cc: Histonet 
Message-ID:
	
Content-Type: TEXT/PLAIN; charset=US-ASCII

Ahhhhh working with a beer at my elbow.  Sounds very sane to me.

I think we got one of your country women..a Wealthal..up for a phd.
She denies dwarf tossing, but I have heard things to the contrary.

I have two square carts with drawers in used in the dental school.
They have wheels on them.  I put the microtome on one and the waterbath on
the other.  They sit side-by side with a space between.  They are just
high enough for me to use a standard height chair with wheels and move
back and forth between them.  It is the best arrangement I have found.  I
can get up over the water bath to get a good look at what is going on.  I
really like it.  Not hard on the back like benches.

Would suggest it for those having problems with benches and sitting still
in one possition.

If I can figure out how to put an ice bucket for the beer between, we's
really have something.
Trisha


On Mon, 17 May 2004, Andrew Kennedy wrote:

> Hi Tim and Fred (and the rest of Histonetters too!!)
>
>
>
> In my experience, we Aussies don't like to stand up at the microtome if we
> can help it. Standing and cutting sections makes no sense at all - we
might
> spill our beer and not be able to see the television that sits on the
bench
> next to us so we can watch Aussie Rules (I prefer Rugby League, by the
way)
>
> :-)
>
> But seriously folks, you would have to have high benches and even then you
> would probably hunch over the microtome - I remember seeing a histotech
> cutting standing up years ago when I was a lab assistant. She turned the
> microtome sideways, stood at the bench (which was desk height) and stooped
> over the microtome. That can't be good for your back! It was the most
> uncomfortable looking cutting position I've ever seen.
>
>
>
> Overlapping with the safety thread that is currently quite "hot", we
really
> owe it to ourselves and to those we work with to be safe all the time!
>
>
>
> In Australia, Occupational Health and Safety is an important issue that
> affects everything we do in the workplace. However, any safety procedure
> that has been implemented in our labs is a result of consultation with the
> parties at stake. Safe Operating Procedures are designed by the people who
> will use them, not by some beaurocrat sitting in an office telling others
> what to do! Take control of your safety and use common sense at the same
> time! Makes sense to me!!
>
>
>
> Andrew Kennedy
>
> Senior Science Officer
>
> Anatomical Pathology
>
> Concord Repatriation General Hospital
>
> Hospital Road
> Concord NSW Australia 2139
>
>
>
> ph: +612 9767 6115
>
> Fax +612 9767 8427
>
>
>
> "oderint dum metuant"
>
>
>
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>



------------------------------

Message: 9
Date: Tue, 18 May 2004 16:29:47 -0400
From: Karen Wittler 
Subject: [Histonet] Congo Red
To: histonet@lists.utsouthwestern.edu
Message-ID: 
Content-Type: text/plain; charset=US-ASCII

 We stain our Congo Reds on an automated stainer now. Before that we
stained with hematoxylin first because rinsing and differentiation was
required and we used an alkaline Congo Red. After staining with the
Congo Red in a saturated sodium chloride- alcohol solution,we did not
rinse,but rapidly dehydrated, cleared and mounted 

 For our H&Es we use Modified Harris Hematoxylin and Eosin-Y w/
Phloxine both manufactured by Richard Allen.

Karen Wittler, HT(ASCP)
Johns Hopkins Hospital
Baltimore, MD



------------------------------

Message: 10
Date: Tue, 18 May 2004 16:16:32 -0500
From: "Ford Royer" 
Subject: Re: [Histonet] MIcrotome alignment
To: KHays@mbhs.org, histonet@lists.utsouthwestern.edu
Cc: Marcia Welch 
Message-ID: <40AA7D30.10601@bitstream.net>
Content-Type: text/plain; charset=us-ascii; format=flowed

On their web site catalog, they show a price of $775.00.

Contact Marcia Welch at: 800-383-7799 or Email: 
 for a customer price quote.

Ford M. Royer, MT(ASCP)
Analytical Instruments, llc
1200 Mendelssohn Ave. N., Ste. 50
Minneapolis, MN 55427
(800) 565-1895, ext. 17
URL: 

KHays@mbhs.org wrote:

>
> Kathy Tedford-Hays  HT (ASCP)
> Technical Specialist, Histology Dept
> (601)-968-3070 ext 7398
> Baptist Medical Center
> 1225 North State Street
> Jackson, MS 39202
>
> how much is it , do you know?
>
>
>
> 	"Ford Royer" 
> Sent by: histonet-bounces@lists.utsouthwestern.edu
>
> 05/18/2004 09:00 AM
>
> 	       
>         To:        histonet@lists.utsouthwestern.edu
>         cc:        
>         Fax to:        
>         Subject:        Re: [Histonet] MIcrotome alignment
>
>
>
>
> Newcomer Supply, Madison, WI sells a microtome aligner.  Product Code 
> 6255A
> Web URL 
> Check it out.
>
> ~ Ford
> Ford M. Royer, MT(ASCP)
> Analytical Instruments, llc
> Minneapolis, MN 55427
> (800) 565-1895, Ext. 17
> 
>
> Roberts, Jon wrote:
>
> >I have recently purchased a Leica RM2255 with the new precise 
> specimen orientation system that adjust to an exact zero position or 
> x-y axis standard.  But I also have old leicas without this system. 
>  Is there any quick and easy way to align my old leicas to the new 
> one????  Just wondering?  I though I've heard of such a device, but 
> wonder if anyone else has heard of it or actually used it?
> >
> >
> >CONFIDENTIALITY NOTICE:  This email message and any accompanying data 
> are confidential, and intended only for the named recipient(s).  If 
> you are not the intended recipient(s), you are hereby notified that 
> the dissemination, distribution, and or copying of this message is 
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> the named recipient(s), please notify the sender at the email address 
> above, delete this email from your computer, and destroy any copies in 
> any form immediately.
> >_______________________________________________
> >Histonet mailing list
> >Histonet@lists.utsouthwestern.edu
> >http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> >
> >  
> >
>
>
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> This internet email has been certified virus free by BHS Anti-Virus 
> system.
>
>


------------------------------

Message: 11
Date: Tue, 18 May 2004 19:59:45 -0400
From: "Mass Histology Service" 
Subject: RE: [Histonet] research charges
To: "Diane M Nelson" ,
	
Message-ID: 
Content-Type: text/plain;	charset="us-ascii"

Diane,

We charge one price across the board, $5.50 if the tissue is received in a
cassette and $2.50 if it needs decalcification.  Our philosophy is that we
don't charge less for simple specimens, so why charge more for occasional
complicated ones? Of course if it's a large project consisting of many whole
eyes or femur heads, I'd probably double our current prices to cover for the
additional labor and material.

Jim

____________________________
  James E. Staruk, HT(ASCP)
   Mass Histology Service
   www.masshistology.com

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Diane M
Nelson
Sent: Tuesday, May 18, 2004 2:04 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] research charges


Hi Histonetters!
Our veterinary lab is in the process of up dating our research charges. I
would like to know what other labs/hospitals charge for H & E bone slides
and H & E eye slides. Currently we are charging, Bone: $19.00 for
decalcifying in 25% formic acid, process, embed, sectioning and H & E
stain. We are losing money on our larger bone projects. Our charges for
processing, embedding sectioning and H & E stain for eye slides is $9.00.
It also depends on the project rather or not we make or lose money. The
researchers do their own grossing of tissue. Any help would be greatly
appreciated! Thanks in advance.

Diane Gerjets
Iowa State University


_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet







------------------------------

Message: 12
Date: Wed, 19 May 2004 11:08:30 +1000
From: Tony Henwood 
Subject: [Histonet] Efficiency of p o and m xylenes in dewaxing
To: "Histonet (Histonet@lists.utsouthwestern.edu)"
	,
"'histonet@pathology.swmed.edu'"
	
Message-ID: <1CF2E2E5BB36D5119E7A0008C791F3740800E1C8@simba.kids>
Content-Type: text/plain

Hi all,

Would anyone have any information on the relative efficiencies of para, meta
and ortho xylenes in dewaxing paraffin sections for nucleic acid extraction.

Does it matter?

Regards

Tony Henwood JP, BAppSc, GradDipSysAnalys, CT(ASC)
Laboratory Manager
The Children's Hospital at Westmead,
Locked Bag 4001, Westmead, 2145, AUSTRALIA.
Tel: 612 9845 3306
Fax: 612 9845 3318




**********************************************************************
This email and any files transmitted with it are confidential and
intended solely for the use of the individual or entity to whom they
are addressed. If you are not the intended recipient, please 
delete it and notify the sender.

Views expressed in this message and any attachments are those 
of the individual sender, and are not necessarily the views of The
Children's Hospital at Westmead

This footnote also confirms that this email message has been 
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the Childrens Hospital at Westmead accepts no liability for any 
consequential damage resulting from email containing computer 
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**********************************************************************



------------------------------

Message: 13
Date: Wed, 19 May 2004 11:08:30 +1000
From: Tony Henwood 
Subject: [Histonet] Efficiency of p o and m xylenes in dewaxing
To: "Histonet (Histonet@lists.utsouthwestern.edu)"
	,
"'histonet@pathology.swmed.edu'"
	
Message-ID: <1CF2E2E5BB36D5119E7A0008C791F3740800E1C8@simba.kids>
Content-Type: text/plain

Hi all,

Would anyone have any information on the relative efficiencies of para, meta
and ortho xylenes in dewaxing paraffin sections for nucleic acid extraction.

Does it matter?

Regards

Tony Henwood JP, BAppSc, GradDipSysAnalys, CT(ASC)
Laboratory Manager
The Children's Hospital at Westmead,
Locked Bag 4001, Westmead, 2145, AUSTRALIA.
Tel: 612 9845 3306
Fax: 612 9845 3318




**********************************************************************
This email and any files transmitted with it are confidential and
intended solely for the use of the individual or entity to whom they
are addressed. If you are not the intended recipient, please 
delete it and notify the sender.

Views expressed in this message and any attachments are those 
of the individual sender, and are not necessarily the views of The
Children's Hospital at Westmead

This footnote also confirms that this email message has been 
virus scanned and although no computer viruses were detected,
the Childrens Hospital at Westmead accepts no liability for any 
consequential damage resulting from email containing computer 
viruses.
**********************************************************************



------------------------------

Message: 14
Date: Wed, 19 May 2004 05:50:52 -0400
From: "Tara Mciver" 
Subject: [Histonet] HTL tissue size minimum requriements 
To: 
Message-ID: 
Content-Type: text/plain;	charset="iso-8859-1"

I attended a readiness workshop in NC and thought I understood the
instructor to say that the tissue sizes should be at least the size
indicated, so actually the tissue size could be larger, and that is what he
suggested we do.  Can anybody verify that I understood him correctly?  Also,
does the tissue have to be a perfect square, or can I just be sure it covers
the square representation provided with the test?  Thanks for any help!




------------------------------

Message: 15
Date: Wed, 19 May 2004 07:11:09 -0400
From: "Weems, Joyce" 
Subject: [Histonet] unsubscribe
To: 
Message-ID:
	
Content-Type: text/plain;  charset="iso-8859-1"

 
 


Confidentiality Notice ** The information contained in this message may be
privileged and is confidential information intended for the use of the
addressee listed above. If you are neither the intended recipient nor the
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information is strictly prohibited. If you have received this communication
in error, please notify us immediately by replying to the message and
deleting it from your computer.
Thank you. Saint Josephs Health System, Inc.


------------------------------

Message: 16
Date: Wed, 19 May 2004 09:08:42 -0400
From: "yan gao" 
Subject: [Histonet] (no subject)
To: histonet@lists.utsouthwestern.edu
Cc: gliuygao@hotmail.com
Message-ID: 
Content-Type: text/plain


   Hi, Everyone.
   I am looking for CD34 antibody stain rat tissues.  Anyone knows where
   I can get it?  Thanks.
     _________________________________________________________________

   [1]Express  yourself  with  the new version of MSN Messenger! Download
   today - it's FREE!

References

   1. http://g.msn.com/8HMBENUS/2752??PS=47575


------------------------------

Message: 17
Date: Wed, 19 May 2004 09:09:28 -0400
From: "yan gao" 
Subject: [Histonet] (no subject)
To: histonet@lists.utsouthwestern.edu
Cc: gliuygao@hotmail.com
Message-ID: 
Content-Type: text/plain


   Hi, Everyone.
   I am looking for CD34 antibody stain rat tissues.  Anyone knows where
   I can get it?  Thanks.

   Yan Gao
   Doctoral of Science
   Novartis
     _________________________________________________________________

   [1]Express  yourself  with  the new version of MSN Messenger! Download
   today - it's FREE!

References

   1. http://g.msn.com/8HMBENUS/2752??PS=47575


------------------------------

Message: 18
Date: Wed, 19 May 2004 09:05:25 -0400
From: "yan gao" 
Subject: [Histonet] (no subject)
To: histonet@list.utsouthwestern.edu, histonet@pathology.swmed.edu
Cc: gliuygao@hotmail.com
Message-ID: 
Content-Type: text/plain


   Hi, Everyone.
   I  am looking for CD34 antibody stain rat tissues.  Anyone knows where
   I can get it?  Thanks.


   Yan Gao

   NOVARTIS
     _________________________________________________________________

   [1]Learn  to  simplify  your finances and your life in Streamline Your
   Life from MSN Money.

References

   1. http://g.msn.com/8HMBENUS/2743??PS=47575


------------------------------

Message: 19
Date: Wed, 19 May 2004 08:10:52 -0500
From: "Galbraith, Joe" 
Subject: RE: [Histonet] HTL tissue size minimum requriements 
To: "Tara Mciver" ,
	
Message-ID:
	<5D03ED7B9391D4119D9B0008C76B7B2403005B1F@uihc-mail1.uihc.uiowa.edu>
Content-Type: text/plain;	charset="iso-8859-1"

The sizes are minimums as you understand.  You will be held strictly
accountable for ensuring that the tissue you submit in your block must
exceed the dimensions listed and that the tissue on the slide must match the
dimensions in the block (without excessive spreading from overfloating on a
warm water bath).  Remember to allow for shrinkage during processing when
you are acquiring your tissue when fixing in formalin and using standard
processing.  To get 2.0 cm of duodenum into the block you may have to cut
2.5 cm from the source tissue depending on your processing schedule.  But
1.9 cm will not due.  Also, if they specify 2.0 cm of duodenum with
epitelium along one side, they mean complete uninterupted epithelium in
perfect condition, perfectly fixed, perfectly processed, perfectly cut, and
perfectly stained.  Any flaws will cost you points.  I don't mean to be
negative just to remind everyone preparing for the practical that you must
pay attention to all the details.  Read the directions very carefully and
follow every picky detail that they specify.  A significant part of the exam
is the applicant's ability to follow written instructions accurately.
(Meaning, if they say a square, I'd recommend a square.)  Pay attention to
the labeling of your slides.  Be sure your labels are clear and readable
(typed), labeled exactly as instructed, and that your label material is in
good condition (stays on the slide, no curls or peeling, etc.)

Hope this helps, good luck.

Joe Galbraith

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Tara
Mciver
Sent: Wednesday, May 19, 2004 4:51 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] HTL tissue size minimum requriements 


I attended a readiness workshop in NC and thought I understood the
instructor to say that the tissue sizes should be at least the size
indicated, so actually the tissue size could be larger, and that is what he
suggested we do.  Can anybody verify that I understood him correctly?  Also,
does the tissue have to be a perfect square, or can I just be sure it covers
the square representation provided with the test?  Thanks for any help!


_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet



------------------------------

Message: 20
Date: Wed, 19 May 2004 09:46:55 -0400
From: phyllis 
Subject: [Histonet] (no subject)
To: histonet@lists.utsouthwestern.edu
Message-ID: <5.1.0.14.0.20040519094604.03b9c2e0@pop.cwru.edu>
Content-Type: text/plain; charset="us-ascii"; format=flowed












Why are you handling this processing? The CJD Surveillance has been set up 
by CDC to do all this work for you and also provide a diagnosis. If your 
pathologists choose to do this themselves, be aware of the protocols which 
can be found at www.cdc.gov and also the WHO website.
The biopsy should be handled in a level 2 facility. You need to wear gloves 
and face shield. All processing and staining is to been done by hand in 
individual containers which will be incinirated on completion, otherwise 
you will have deal with the demanding work of decontaminating the processor.
All waste is incinerated.
You will find all this info at CDC website. Also refer to 
www.cjdsurveillance.com if you decide to let the CJD Survellance do this 
for you. Free of charge.


Phyllis Scalzo, HT(ASCP)

Case Western Reserve University
2085 Adelbert Rd.
Cleveland, Ohio 44106
Ph: 216-368-0822
Fax: 216-368-2546




------------------------------

Message: 21
Date: Wed, 19 May 2004 07:48:52 -0600
From: "Connie McManus" 
Subject: RE: [Histonet] H&E stain problems
To: "'Gary Gill'" , "'Marshall Terry Dr,	Consultant
	Histopathologist'" , 	"'Petia P
	Stefanova'" ,	"'Megan
Kear'"
	,
	
Cc: histonet@lists.utsouthwestern.edu
Message-ID: <000a01c43da8$08034a90$4a737b81@Cygnus>
Content-Type: text/plain;	charset="us-ascii"

Gary

Is Gill a relative or yours???  I don't give a hoot who made what when.
I like Harris.  I don't believe Gill has made any significant
improvements over the old dead white guy's hematoxylin.  

BTW, classical music may have been composed by dead white guys, but I
don't hear ANYTHING being composed today that has the depth, complexity
and color of those great composers.  I've studied piano from since I was
4 yrs old, and I've studied the organ (I've played as a church
organist)for almost 20  years.  I know music like the back of my hand
and I love ALL music (except gangstah stuff). SO DON'T try to tell me
about what music is living and worth my while.  

Dead white Europeans. Yeah, right. I'd like to see someone between 8 and
14 in this generation compose music like Felix Mendelsohnn's or
Mozart's. 

Connie McManus
Utah Veterinary Diagnostics Laboratory
Utah State University
Logan, UT
Phone:  435/797-1891
fax: 435/797-2805


-----Original Message-----
From: Gary Gill [mailto:garygill@dcla.com] 
Sent: Tuesday, May 18, 2004 10:04 AM
To: 'Connie McManus'; 'Marshall Terry Dr,Consultant Histopathologist';
'Petia P Stefanova'; 'Megan Kear'; histonet@lists.utsouthwestern.edu
Cc: histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] H&E stain problems

You may have heard that classical music was composed by dead white
Europeans.  Well, Harris hematoxylin was composed by a dead white
American
(physician at Jefferson Hospital in 1904).  Gill hematoxylin was
composed by
a live white American.  So if you want to liven things up, go with
Gill's!

Gary Gill (one and the same)

PS -- No royalties involved, thanks to bad advice in 1972 from corporate
counsel for Johns Hopkins Medical School.

-----Original Message-----
From: Connie McManus [mailto:convmcm@cc.usu.edu] 
Sent: Tuesday, May 18, 2004 10:41 AM
To: 'Marshall Terry Dr,Consultant Histopathologist'; 'Petia P
Stefanova';
'Megan Kear'; histonet@lists.utsouthwestern.edu
Cc: histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] H&E stain problems


Wow.  What a lot of interesting comments!!  

I agree with Terry re the agitation.  When I watch the stainer do those
dips
(I can program how many, but NOT the briskness), I wonder if you could
even
call it agitation.  My hand dips are very brisk.  Also, I don't bother
letting the slides stay in the alcohols for 1 minute or 2, I give the
slides
about 20 -30 good brisk dips in each solution, then the timed rinses &
staining.  This has always been far more satisfactory to me than those
sllllooooowwwww dips from the machine.

As for the kind of hematoxylin, someone suggested I throw out the Harris
and
do Gills III.  I've tried Gill III before and I much prefer the Harris.
So
it's just a matter of personal preference on that... AND what your
pathologist likes *G*

Everyone having an nice Tuesday??? *g*

Connie McManus
Utah Veterinary Diagnostics Laboratory
Utah State University
Logan, UT
Phone:  435/797-1891
fax: 435/797-2805


-----Original Message-----
From: Marshall Terry Dr, Consultant Histopathologist
[mailto:Terry.Marshall@rothgen.nhs.uk] 
Sent: Tuesday, May 18, 2004 7:35 AM
To: Connie McManus; Petia P Stefanova; Megan Kear;
histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] H&E stain problems

Connie remarks:

"In truth, I prefer my hand stained sections better than when they're
stained automatically."

When I first saw what I call x-y stainers, I thought that we had in
this,
something that reproduced hand staining. Not so. What I think is lacking
is
the brisk agitation necessary to break down the interface between old
and
new solution. I'm not so sure about the rinsing either.

Dr Terry L Marshall, B.A.(Law), M.B.,Ch.B.,F.R.C.Path  Consultant
Pathologist  Rotherham General Hospital  South Yorkshire  England
        terry.marshall@rothgen.nhs.uk

-----Original Message-----
From: Connie McManus [mailto:convmcm@cc.usu.edu]
Sent: 18 May 2004 15:09
To: 'Petia P Stefanova'; 'Megan Kear'; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] H&E stain problems


We use almost the exact same protocol... we use Surgipath Harris, but we
prepare eosin in-house.  One thing I am amazed at in this protocol is
the
length of time in the acid alcohol.  Do you use an autostainer?  We have
a
Leica.  The time is set to 1 second in the acid ETOH and
sometimes the sections are almost too differentiated.   I can't imagine
6 seconds in the acid ETOH!!  Even when I do H&E manually, I dip the
slides
in and quickly put them in running water.  I must have a very strong
solution, I guess. Hmmmm. Interesting.  In truth, I prefer my hand
stained
sections better than when they're stained automatically.

Just wondering and blabbering (hey, it's Tuesday, what do you expect??)

Connie McManus
Utah Veterinary Diagnostics Laboratory
Utah State University
Logan, UT
Phone:  435/797-1891
fax: 435/797-2805


-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Petia P
Stefanova
Sent: Monday, May 17, 2004 6:45 AM
To: Megan Kear; histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] H&E stain problems

Hi,

I use Harris's hematoxylin which is also regressive and purchase my
hematoxylin and eosin /alcohol-based/ from www.surgipath.com. I get very
good H&E staining with this protocol.

REAGENT                       TIME
Xylene                                3 min.
Xylene                                3 min.
Abs. alc.                              2 min.
Abs. alc.                              2 min
95% alc.                              2 min
80% alc.                              2 min
Wash /tap water/                  30 sec.
Hematoxylin                          8 min.
Wash /tap water/                  2 min.
Acid alcohol                          6 sec.
Wash /tap water/                  2 min.
Wash /tap water/                  5 min
80% alc.                              30 sec.
Eosin                                    15 sec.
95% alc.                              10 sec.
Abs. alc.                              30 sec.
Abs. alc.                              30 sec.
Xylene                                1 min.
Xylene                                1 min.
Xylene                                Exit

Hope it helps!
Petia


_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet


_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet


_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet




------------------------------

Message: 22
Date: Wed, 19 May 2004 14:56:37 +0100
From: "Marshall Terry Dr,	Consultant Histopathologist"
	
Subject: RE: [Histonet] H&E stain problems
To: "Connie McManus" , "Gary Gill"
	,	"Petia P Stefanova"
	,	"Megan Kear"
	,
	
Cc: histonet@lists.utsouthwestern.edu
Message-ID:
	
Content-Type: text/plain;	charset="iso-8859-1"

Close relative:-)

Dr Terry L Marshall, B.A.(Law), M.B.,Ch.B.,F.R.C.Path
 Consultant Pathologist
 Rotherham General Hospital
 South Yorkshire
 England
        terry.marshall@rothgen.nhs.uk

-----Original Message-----
From: Connie McManus [mailto:convmcm@cc.usu.edu]
Sent: 19 May 2004 14:49
To: 'Gary Gill'; Marshall Terry Dr, Consultant Histopathologist; 'Petia
P Stefanova'; 'Megan Kear'; histonet@lists.utsouthwestern.edu
Cc: histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] H&E stain problems


Gary

Is Gill a relative or yours???  I don't give a hoot who made what when.
I like Harris.  I don't believe Gill has made any significant
improvements over the old dead white guy's hematoxylin.  

BTW, classical music may have been composed by dead white guys, but I
don't hear ANYTHING being composed today that has the depth, complexity
and color of those great composers.  I've studied piano from since I was
4 yrs old, and I've studied the organ (I've played as a church
organist)for almost 20  years.  I know music like the back of my hand
and I love ALL music (except gangstah stuff). SO DON'T try to tell me
about what music is living and worth my while.  

Dead white Europeans. Yeah, right. I'd like to see someone between 8 and
14 in this generation compose music like Felix Mendelsohnn's or
Mozart's. 

Connie McManus
Utah Veterinary Diagnostics Laboratory
Utah State University
Logan, UT
Phone:  435/797-1891
fax: 435/797-2805


-----Original Message-----
From: Gary Gill [mailto:garygill@dcla.com] 
Sent: Tuesday, May 18, 2004 10:04 AM
To: 'Connie McManus'; 'Marshall Terry Dr,Consultant Histopathologist';
'Petia P Stefanova'; 'Megan Kear'; histonet@lists.utsouthwestern.edu
Cc: histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] H&E stain problems

You may have heard that classical music was composed by dead white
Europeans.  Well, Harris hematoxylin was composed by a dead white
American
(physician at Jefferson Hospital in 1904).  Gill hematoxylin was
composed by
a live white American.  So if you want to liven things up, go with
Gill's!

Gary Gill (one and the same)

PS -- No royalties involved, thanks to bad advice in 1972 from corporate
counsel for Johns Hopkins Medical School.

-----Original Message-----
From: Connie McManus [mailto:convmcm@cc.usu.edu] 
Sent: Tuesday, May 18, 2004 10:41 AM
To: 'Marshall Terry Dr,Consultant Histopathologist'; 'Petia P
Stefanova';
'Megan Kear'; histonet@lists.utsouthwestern.edu
Cc: histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] H&E stain problems


Wow.  What a lot of interesting comments!!  

I agree with Terry re the agitation.  When I watch the stainer do those
dips
(I can program how many, but NOT the briskness), I wonder if you could
even
call it agitation.  My hand dips are very brisk.  Also, I don't bother
letting the slides stay in the alcohols for 1 minute or 2, I give the
slides
about 20 -30 good brisk dips in each solution, then the timed rinses &
staining.  This has always been far more satisfactory to me than those
sllllooooowwwww dips from the machine.

As for the kind of hematoxylin, someone suggested I throw out the Harris
and
do Gills III.  I've tried Gill III before and I much prefer the Harris.
So
it's just a matter of personal preference on that... AND what your
pathologist likes *G*

Everyone having an nice Tuesday??? *g*

Connie McManus
Utah Veterinary Diagnostics Laboratory
Utah State University
Logan, UT
Phone:  435/797-1891
fax: 435/797-2805


-----Original Message-----
From: Marshall Terry Dr, Consultant Histopathologist
[mailto:Terry.Marshall@rothgen.nhs.uk] 
Sent: Tuesday, May 18, 2004 7:35 AM
To: Connie McManus; Petia P Stefanova; Megan Kear;
histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] H&E stain problems

Connie remarks:

"In truth, I prefer my hand stained sections better than when they're
stained automatically."

When I first saw what I call x-y stainers, I thought that we had in
this,
something that reproduced hand staining. Not so. What I think is lacking
is
the brisk agitation necessary to break down the interface between old
and
new solution. I'm not so sure about the rinsing either.

Dr Terry L Marshall, B.A.(Law), M.B.,Ch.B.,F.R.C.Path  Consultant
Pathologist  Rotherham General Hospital  South Yorkshire  England
        terry.marshall@rothgen.nhs.uk

-----Original Message-----
From: Connie McManus [mailto:convmcm@cc.usu.edu]
Sent: 18 May 2004 15:09
To: 'Petia P Stefanova'; 'Megan Kear'; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] H&E stain problems


We use almost the exact same protocol... we use Surgipath Harris, but we
prepare eosin in-house.  One thing I am amazed at in this protocol is
the
length of time in the acid alcohol.  Do you use an autostainer?  We have
a
Leica.  The time is set to 1 second in the acid ETOH and
sometimes the sections are almost too differentiated.   I can't imagine
6 seconds in the acid ETOH!!  Even when I do H&E manually, I dip the
slides
in and quickly put them in running water.  I must have a very strong
solution, I guess. Hmmmm. Interesting.  In truth, I prefer my hand
stained
sections better than when they're stained automatically.

Just wondering and blabbering (hey, it's Tuesday, what do you expect??)

Connie McManus
Utah Veterinary Diagnostics Laboratory
Utah State University
Logan, UT
Phone:  435/797-1891
fax: 435/797-2805


-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Petia P
Stefanova
Sent: Monday, May 17, 2004 6:45 AM
To: Megan Kear; histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] H&E stain problems

Hi,

I use Harris's hematoxylin which is also regressive and purchase my
hematoxylin and eosin /alcohol-based/ from www.surgipath.com. I get very
good H&E staining with this protocol.

REAGENT                       TIME
Xylene                                3 min.
Xylene                                3 min.
Abs. alc.                              2 min.
Abs. alc.                              2 min
95% alc.                              2 min
80% alc.                              2 min
Wash /tap water/                  30 sec.
Hematoxylin                          8 min.
Wash /tap water/                  2 min.
Acid alcohol                          6 sec.
Wash /tap water/                  2 min.
Wash /tap water/                  5 min
80% alc.                              30 sec.
Eosin                                    15 sec.
95% alc.                              10 sec.
Abs. alc.                              30 sec.
Abs. alc.                              30 sec.
Xylene                                1 min.
Xylene                                1 min.
Xylene                                Exit

Hope it helps!
Petia


_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet


_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet


_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet




------------------------------

Message: 23
Date: Wed, 19 May 2004 08:03:16 -0600
From: "Connie McManus" 
Subject: RE: [Histonet] CJD DECONTAMINATION PROCEDURE
To: , 
Message-ID: <000c01c43daa$087de6e0$4a737b81@Cygnus>
Content-Type: text/plain;	charset="us-ascii"

I don't deal with CJD, but I do a lot of CWD (Chronic Wasting Disease)
and Scrapie, which are prion diseases in dear/elk and sheep
(respectively).  CWD and Scrapie had been associated with humans for
hundreds of years with no known transmission of the disease from animal
to human. HOWEVER, since prions are not neutralized by fixation of any
kind, not killed by autoclaving, this makes them a biohazard in
processed tissues.  

If I worked with CJD, I would ...
1  set up a place that is completely separate from the rest of the lab.
Barrier off a section of your lab, use another room (preferable)

2 use disposable bunny suits, shoe covers, face masks, eye wear and
DOUBLE GLOVE 
3  put stickie mats on the floor completely surrounding the cutting
area,

4   put ALL paraffin shavings in a biohaz bag, 

5  use paper towels to clean off the microtome and throw those in the
biohaz bag

6. incinerate the bunny suit, goggles, gloves, face maskes, etc with the
paraffin shavings.

7.  Ask for a really big pay raise.  Claim it's hazard pay.

Have fun.
Connie McManus
Utah Veterinary Diagnostics Laboratory
Utah State University
Logan, UT
Phone:  435/797-1891
fax: 435/797-2805


-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of
srishan@mail.holyname.org
Sent: Tuesday, May 18, 2004 9:59 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] CJD DECONTAMINATION PROCEDURE





Hello everyone,

This question might have been posted before, but,   Histology Department
is
expecting a brain biopsy from a possible CJD patient today.  The
specimen
will be coming down in formalin from the O.R.  It will be kept in
formalin
for 2-7 days and will be transferred to formic acid for the required
time.
Then the specimen will be transferred to formalin before processing for
another 2 days.  Has anyone dealt with this procedure before. These are
the
questions asked by the hstology department.

How do you decontaminate the processor, microtome, stainer and
coverslipper?
What kind of protective gear apart from standard precaution has to be
used?
How about the shaving of the tissues?
How do you dispose the formalin  from the original formalin container
where
the specimen was sent in and how do you dispose the reagents from the
processor and the stainer?
Can this biopsy be processed, once treated with formic acid, along with
the
other specimens or  should the biopsy be processed with the other
specimens?

Need Help!!

Thank you in advance

Mala Srishan
Histology Supervisor
Holy Name Hospital
Teaneck, NJ 07666


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End of Histonet Digest, Vol 6, Issue 28
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