Good Morning, Histonetters,
I am hoping for some suggestions.  I have just run VEGF(Santa Cruz mab sc-7269) on FFPE rat brains.  I pretreated with RTU proteinase K from Biogenex, and diluted my antibody to 1:300 and incubated for 45 mins. at room temp.  In previous runs with the same conditions, I have gotten beautiful staining on a human hemangiosarcoma specimen.
In these rat brains, which are controls for an epilepsy study, EVERY neuron has stained positive for VEGF.  There is no background, and the stain is cytoplasmic in EVERY neuron.
Does anyone have any experience with this phenomenon?  I know there has been much discussion about VEGF antibodies and everyone seemed to agree that the Santa Cruz monoclonal was the best, which is my experience as well.
Thanks for any thoughts.
Nancy Lemke 
Hermelin Brain Tumor Center
Henry Ford Hospital
Detroit

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