[Histonet] Research Diagnstics CD 66

From:Melanie Black

Hi There

I am running an antibody from Research Diagnostics - CD 66 
(Granulocyte Marker).Has anybody had success with it on formalin 
fixed tissue, and is so, what antigen retrieval have you found to be 
successful. I have had no problem with it on unfixed blood smears, 
BUT no luck on fixed tissue.

My antibody is RDI-CD66B-G10F5, and is recommended for formalin fixed 
tissue, with citrate pre treatment. It also requires an IgM 
secondary, which I have used!I have tries citrate buffer as well as 

Any suggestions???

Many Thanks
Melanie Black.
Cardiovascular Research Unit
Div. of Cardiothoracic Surgery
Chris Barnard Building
University of Cape Town
Anzio Road
Republic of South Africa

Tel +27 21 406-6589
Cel +27 82 469-3352
Fax +27 21 448-5935
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