[Histonet] Re: Frozen sections

From:"Johnson, Teri"

Because of the "thaw" schedule of most cryostats, storing a block of
tissue in the cryostat overnight is not a good practice.  If your
pathologist is worried about losing orientation of the tissue (or
whatever the rationale is...) keep the sample on the chuck, wrap it in
foil, and store it in a -20 to -80C freezer overnight.  If you use the
-20C freezer, make absolutely certain it is not a frost-free model.
Otherwise you can cut your slides in advance and store the remainder of
unused slides in the freezer and take out what is necessary when you
need them.

Teri Johnson
Managing Director Histology Facility
Stowers Institute for Medical Research
1000 E. 50th St.
Kansas City, Missouri  64110

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