[Histonet] RE: Fixing frozens in 4% PFA
I frequently use an immunohistochemistry protocol which calls for BRIEF fixation of the frozen slides in PFA or 10% Neutral buffered Formalin (30sec up to 5min). This seems to help adhere the sections to the slide more so than have any additive effect on the tissue. I 've never played with the fixation times to see at what point IHC signal is affected. Good Luck!
One MedImmune Way
Gaithersburg, MD 20878
Date: Wed, 12 May 2004 11:35:59 -0500
From: NIDAL E MUVARAK
Subject: [Histonet] Fixing frozen sections in 4% Paraformaldehyde.
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Is it common to fix frozen sections of unfixed tissue with 4% PFA the same way they can be fixed with acetone? I know it will definitely take longer than aceton fixation, but has that been done? Thanks.
Nidal E Muvarak
Associate Research Specialist
Vascular Tissue Biomechanics Laboratory
Department of Biomedical Engineering
University of Wisconsin-Madison
1550 Engineering Dr.; Rm. 2158
Madison, WI 53706-1609
Lab: (608) 265-8921; Office: (608) 265-4205;
Home: (608) 256-7934; Cell: (608) 332-6068
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