[Histonet] RE: Fixing frozens in 4% PFA

From:"Wester, Martha"

I frequently use an immunohistochemistry protocol which calls for BRIEF fixation of the frozen slides in PFA or 10% Neutral buffered Formalin (30sec up to 5min).  This seems to help adhere the sections to the slide more so than have any additive effect on the tissue.  I 've never played with the fixation times to see at what point IHC signal is affected.  Good Luck!
Martha Wester
Associate Scientist
MedImmune, Inc.
One MedImmune Way
Gaithersburg, MD 20878
fax 301-398-9794

Message: 13
Date: Wed, 12 May 2004 11:35:59 -0500
Subject: [Histonet] Fixing frozen sections in 4% Paraformaldehyde.
To: histonet@pathology.swmed.edu
Message-ID: <28870a286898.28689828870a@wiscmail.wisc.edu>
Content-Type: text/plain; charset=us-ascii

Is it common to fix frozen sections of unfixed tissue with 4% PFA the same way they can be fixed with acetone? I know it will definitely take longer than aceton fixation, but has that been done? Thanks.

Nidal E Muvarak
Associate Research Specialist
Vascular Tissue Biomechanics Laboratory
Department of Biomedical Engineering
University of Wisconsin-Madison
1550 Engineering Dr.; Rm. 2158
Madison, WI 53706-1609
Lab: (608) 265-8921; Office: (608) 265-4205; 
Home: (608) 256-7934; Cell: (608) 332-6068

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