Im in the validation process for EGFR. I have noticed that the normal liver cells in liver core biopsies have a positive membrane stain, 2+ - 3+ . The metastic tumor cells are not difficult to identify, since their membrane staining when positive is not nearly as uniform or as intense as the normal liver cells. Has anyone else seen this? No other normal cells in the tissues we have stained stains positive. We are using Ventana antibody on the Benchmark and following the recommend protocol.
Baptist Hospital of E TN
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