I am trying to do IHC with Cox -2 ,The protocol I have is a long overnight
incubation with TSA kit.
I am trying to reduce the time of incubation using Dako's LSAB+  system or
LSAB2 .
Please pass me any protocol which can be helpful.

Thank you in advance.

Moumita Kundu
----- Original Message ----- 
From: "Elizabeth Chlipala" 
To: "'Histonet'" 
Sent: Tuesday, May 11, 2004 10:33 AM
Subject: [Histonet] ED-A fibronectin antibody in rat


> Hello
>
> I need to perform immunohistochemistry for ED-A fibronectin on rat
> kidneys.  I have done a bit of searching on the internet and have only
> been able to locate one source for this antibody.  Its from Abcam
> (ab6328)  from what I get off their web page is that this antibody will
> work on frozen sections, but there is no information available on
> formalin fixed paraffin embedded tissues.  Does anyone know if this
> antibody will work on FFPE tissues and is there another source for this
> antibody other than Abcam that will work in rat?  Thanks in advance.
>
> Liz
>
> Elizabeth A. Chlipala, BS, HTL(ASCP)
> Premier Histology Laboratory, LLC
> P.O. Box 18592
> Boulder, Colorado 80308
> Office: (303) 735-5001
> Fax: (303) 735-3540
> lizchlipala@premierhistology.com
> www.premierhistology.com
>
> Ship to Address:
> Premier Histology Laboratory
> University of Colorado
> MCBD, Room A3B40
> Boulder, Colorado 80309
>
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