[Histonet] BDNF IHC

From:Robert Schoonhoven

Fellow 'Netter's,

I've been having some problems with anti-human BDNF IHC.  The clone is 
MAB248 from R&D Systems.  The problem: no staining.

Solutions tried: Different titrations, incubation time and temp. as well 
as HEIR (also tried calling their tech service -nada)

Question: Who is doing this procedure and what are you doing that makes 
it work?   What antibody are you suggesting (use).

TIA

Robert Schoonhoven,
Center for Environmental Health and Susceptibility
& Lab. of Molecular Carcinogenesis and Mutagenesis
UNC-CH
919.966.6343


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