casting resin

From:katherine-walters@uiowa.edu



Hi- here's a weird one.  We were using our sliding microtome on a whole mouse 
and the P.I. got excited and asked if we could preserve the slices and even a 
half cut mouse in casting resin.  I got some and tried it.  I fixed the mouse,
putting a slide on top to keep the organs in place, rinsed it in buffer and 
soaked it in the casting solution a few minutes.  Then I removed it to a mold 
containing the casting solution plus hardner.  It hardened, but is a little 
sticky and not as smooth on the outside as I would have liked.  Also the organs 
all turned a paler shade of white.  Are there protocols and/or stains that 
would help with something like this?

Thanks for any imput!!

Kathy  




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