To my fellow histoneters

I am trying to stain Rat MCL with the ABC staining kit.  My primary antibody 
is collagen 1 and then I use the kit for the rest of the reagents.  standard 
secondary and then so on.  My question is that when I get down to the 
peroxidase step, the tissue does not change' color all that much.  When 
viewed under the microscope its a grey color.  I talked to Santa Cruz and 
they said that it could be that the DAB Chromagen in the kit is percipating 
out.

If anyone' has stained using the ABC staining kit from SANTA CRUZ, and has 
any suggestions for me let me know.
>
>>
>Andrew
UH TECH
>
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