Diana,
we place 100 mLs of  1% alcoholic Eosin in our first 100% alcohol on the
processor and it works well.  We process alot of cervical biopsies and I
agree, it's hard to see anything at all at 3:30 AM, never mind trying to
find white tissue surrounded by white paraffin.

Joe Nocito, BS, HT (ASCP) QIHC
Histology Supervisor
Pathology Reference Lab
San Antonio, TX
jnocito@pathreflab.com
----- Original Message -----
From: Diana McCaig 
To: HistoNet Server 
Sent: Wednesday, May 21, 2003 8:15 AM
Subject: Pre staining white biopsies for easy visibility in paraffin block s


> I know this issue was addressed previously but would like some additional
> assistance.  Years back I worked in a lab that colored their biopsies at
the
> grossing bench with an eosin solution.  I can not recall the strength or
> concentration but for some reason a saturated alcoholic solution sounds
> familiar.  I have tried this as well as adding 20 drops of 5% eosin/200 ml
> to the first alcohol (75%) on our RMC processor.  But, neither method
> colored the biopsies.  I do not want to use mercurichrome or methylene
blue.
> I want to use an eosin based solution.  Can anyone provide me with a
recipe
> of success.  I would prefer coloring them at the grossing bench rather
than
> in the processor but will try anything that will make the biopsies
visible.
> I don't like cutting by brail.
> Diana McCaig, R.T.
> Charge Tech, Histology
> Chatham Kent Health Alliance
> 519-352-6401 (3347)
>
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