Re: JK's reply on section adhesives

From:carl hobbs

John, can you definitively state what type of adhesive Merck/VWR use on the
Superfrost plus slides they sell? I ask in context of AG retrieval on FFPW
sections. Before silane- coating became the norm, I used to use "subbing
solution" but then found silane better( I used to double-sub for
skin/cartilage tissues, for example, to obtain good adhesion thro the M/W
process). Any other adhesive was useless. I compared silane and Superfrost
plus for quite a while before opting for the (  more expensive) latter.
Gayle Callis has suggested that they also use silane but, if so, perhaps
their slides are more rigourously cleaned, to give the better adhesion for
"difficult " tissues?
NB. I have used the range of "buffers" cited for use in AG retrieval.
Interestingly, using TRIS or EDTA at pH8-9 resulted in section loss yet,
10Mm TRIS pH10 is superb in many cases( also endogenous biotin is "wiped"
out, unlike when using citric pH6). As pH10 is most definitely alkaline, how
come the Superfrost slides are still excellent in keeping sections on the
slide? I have to also admit that citric at pH6 sometimes results in loss of
section detail sometimes, but not always. Any ideas why? I don't get this
with TRISpH10 at all( mind you, some Ags need citric pH6, not
TRISpH10.........TRISpH6 is no good for me)
    Off subject, may I state that your Histotech book is the most
informative/invaluable. It follows in the tradition of the  books that
inform practice, that began with Drury and Wallington, then Disbrey and
Wrack ( please forgive any spelling mistakes, it's from memory). It is also
the most  enjoyable, apposite to current practise, book to read( as are your
posts). It would be interesting to read a response from Russ Allison,
another of my most respected Histotechs
PS Can I borrow a fiver? Lol
PPS
Anyone wanting to cast their Qs wider may well be rewarded by posting,
additionally to Hogne's site at http://home.no.net/immuno/discussion.html No
pop-ups!
>


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