Hi!

The problem is with your lead citrate. Make up a new batch. We only make a 
small batch and make it up more frequently.

Tammy Freeman
QEII Health Sciences Centre
Nova Scotia, Canada


>From: sara goldston 
>To: histonet@pathology.swmed.edu
>Subject: EM Staining questions
>Date: Wed, 21 May 2003 05:57:12 -0700 (PDT)
>
>I work in electron microscopy. Lately, staining has been a problem. 
>Sections have exhibiting poor contrast and/or considerable black deposits. 
>In our lab, we utilize water-based and methylene-based uranyl acetate, 
>Reynold's and Sato's lead citrate. In particular, the methylene UA
>
>
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