Re: Breast Tissue
Dear Roxanne, Your email to Histonet does not
say who you are or for whom you work. Please
reveal your position in relation to
"rapid fix from BBC."
Please will you tell all of us
what "rapid fix from BBC" is, and
_why_ anybody should try it out
on fatty breast tissue.
The principles of fixing different kinds of
specimens for frozen sections or parsffin
procesing were well known 101 years ago
(Gustav Mann's "Physiological Histology." 1902;
a good read if you have an old library). The
chemistry of fixation is understood better now
than in Mann's time, but old Gus had all the right
ideas.
Fixation determines the microanatomy and cytology
seen by the pathologist, tecnician or computer
trying to make a diagnosis. Just imagine yourself
being interrogated by a barrister and having to
admit that you did not know how the fixative
affected the diagnosis. Why should any of us
risk using a "rapid fix from BBC" without
knowing what it is and if or how it works?
There are plenty of thoroughly understood fixative
mixtures that work well. Thers is no place for
the secret brew.
--
------
John A. Kiernan
Department of Anatomy and Cell Biology
The University of Western Ontario
London, Canada N6A 5C1
kiernan@uwo.ca
http://publish.uwo.ca/~jkiernan/
-------------
GREYTRUNK@aol.com wrote:
>
> Try rapid fix from BBC.
> Roxanne
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