Re: Breast Tissue

From:"J. A. Kiernan"

Dear Roxanne,  Your email to Histonet does not 
say who you are or for whom you work. Please
reveal your position in relation to
"rapid fix from BBC."

Please will you tell all of us 
what "rapid fix from BBC" is, and
_why_ anybody should try it out
on fatty breast tissue.

The principles of fixing different kinds of
specimens for frozen sections or parsffin 
procesing were well known 101 years ago
(Gustav Mann's "Physiological Histology." 1902;
a good read if you have an old library). The
chemistry of fixation is understood better now
than in Mann's time, but old Gus had all the right
ideas.

Fixation determines the microanatomy and cytology
seen by the pathologist, tecnician or computer
trying to make a diagnosis. Just imagine yourself 
being interrogated by a barrister and having to 
admit that you did not know how the fixative 
affected the diagnosis. Why should any of us
risk using a "rapid fix from BBC" without 
knowing what it is and if or how it works?

There are plenty of thoroughly understood fixative
mixtures that work well. Thers is no place for
the secret brew.
--
------
John A. Kiernan
Department of Anatomy and Cell Biology
The University of Western Ontario
London,   Canada   N6A 5C1
   kiernan@uwo.ca
   http://publish.uwo.ca/~jkiernan/
-------------
GREYTRUNK@aol.com wrote:
> 
> Try rapid fix from BBC.
> Roxanne
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