RE: confocal

From:Patsy Ruegg

Cynthia,
eosin fluoreses but best used aqueous at low concentration ?1%, but I do not
know if it will stain your borellia organism.  i use it's fluorescent
quality to stain osteoid and then look at it under UV, it is brilliant.
alcoholic eosin get all over and is not specific in my hands.
Patsy

-----Original Message-----
From: Favara, Cynthia (NIH/NIAID) [mailto:cfavara@niaid.nih.gov]
Sent: Tuesday, May 20, 2003 7:41 AM
To: HistoNet Server (E-mail)
Subject: confocal


All,

A post doc came by yesterday with a question. I am asking for input.

She has removed Borellia infected salivary glands from mice, fixed with
acetone and put on slides. She now wants to do Immuno-flourescence using the
confocal [ She has a tagged antibody for the Borellia]  She would like to
visualize the salivary gland to see if the Borellia is within cells or
external. I recall some talk of hematoxylin being fluorescent and I think
acridine orange is as well. I have no idea if this will work but she would
like to try something before she starts all over again with frozen or
possible vibratome sections. On searching the archives I did not find
anything encouraging. John Kiernan had some comments and I will check his
book.

Anyone out there with suggestions please pass them on.

Please resist suggesting they check before staring a project as I believe
that is useless here!

C

Cynthia Favara
NIAID/NIH/RML/LPVD
903 South 4th Street
Hamilton, MT 59840
406-363-9317





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