RE: Skins and IF's
Deb:
We
occassionally thaw a skin that has been misoriented when (and if) it was
embedded in OCT at the time of freezing. Generally thawing and refreezing
would not be the prefered method of handling due to potential for ice
artifact, reduction in antigenicity, chance for separation of epidermal
layer, etc. But if handled correctly and properly refrozen, it can
be done.
Good
luck,
Joe
Galbraith
Say...can
anyone tell me if skin's that are flash frozen and stained for
immunoflourescence can be thawed and refrozen? I know muscle can because
of freeze artifact but can skin?
Thanks:-)
Deb King, HT
Sacramento, CA
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