RE: DAB removal
Nick,
We use the DABOut system from Cardinal (Allegiance).
Donna Willis
Histology Lab Manager
Harris Methodist Fort Worth, Tx
-----Original Message-----
From: K. Bowden [mailto:kbowden@ucsd.edu]
Sent: Wednesday, May 21, 2003 11:43 AM
To: Nick_Madary@hgsi.com
Cc: histonet@pathology.swmed.edu
Subject: Re: DAB removal
Hi Nick
Here is a method we use for DAB disposal. Most of us feel
that it is easier to just collect it and let our
Environment, Health and Safety Department pick it up.
DAB DISPOSAL
DAB (Diaminobenzidine tetrahydrochloride). Carcinogenic
determination: neoplastic by RTECS criteria following
chronic feeding studies in mice (tumors in the respiratory
system).
DISPOSAL: The practice of treating waste solutions with
chlorine bleach (sodium hypochlorite or Cloroxo) is not
recommended, as the outcome of the reaction is unknown.
According to Lunn & Sansone (Destruction of Hazardous
Chemicals).
1. Prepare the following aqueous stock solutions.
a. 0.2 M potassium permanganate
Potassium permanganate(KMnO4) 31.6 g
Deionized water 1000 ml
b. 2.0 M sulfuric acid
Sulfuric acid (concentrated) 112 ml
Deionized water 1000 ml
2. Dilute the DAB solution if necessary so that its
concentration does not exceed 0.9 mg/ml.
3. For each 10 ml of DAB solution, add:
a. 5 ml of solution 1.a.
b. 5 ml of solution 1.b.
4. Allow mixture to stand for at least 10 hrs. It is now
non-mutagenic.
5. Decolorize the mixture with ascorbic acid (add powder
until color disappears).
6. Neutralize the decolorized mixture with sodium
bicarbonate (test with pH meter, pH paper or dipsticks)
7. Discard the solution down the drain provided that local
waste water authorities have given their approval.
--
Karen Bowden
Staff Research Associate II
University of CA, San Diego
Department of Orthopedics
9500 Gilman Dr. 0630
La Jolla, CA 92093-0630
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Nick_Madary@hgsi.com wrote:
>
> Any quick answers on removal of DAB? We are using bleach to
> "neutralize" I know that is not the correct phrase, and then we place in
> a container to have it removed. Is there a problem with pouring it down
> the sink? Is there another accepted method of removal?
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