Joey,
CAE can be affected by fixation, processing and decalcification.  This is
why we always did CAE on smears not processed tissue.
Patsy

-----Original Message-----
From: Joey Shepherd [mailto:j.shepherd@sheffield.ac.uk]
Sent: Friday, May 23, 2003 4:09 AM
To: histonet@pathology.swmed.edu
Subject: Chloroacetate esterase


Morning all
I've been using Sigma's Naphthol AS-D chloroacetate esterase kit to
stain arthritic mouse joints and surprisingly no neutrophils are
staining up (although you can see they're there on H&E) but the mast
cells are staining beautifully! I was wondering if the
decalcification process (EDTA) affects the esterase activity of
neutrophils but not mast cells? oes anyone have any ideas? It's not
too much of a disaster, more a curiosity.
Cheers!
Joey
--
Dr Joey Shepherd
Postdoctoral scientist
Academic Unit of Genetics and Informatics
Division of Genomic Medicine
The University of Sheffield Medical School
M Floor, Royal Hallamshire Hospital
Sheffield S10 2JF
UK

Phone 0114 271-3007
Fax 0114-272-1104