I would be interested in a processing schedule for cell pellets also.

Thank you 

Gina Sennello

Associate Scientist
OSI Pharmaceuticals
Boulder, CO




-----Original Message-----
From: Crosby, Katherine [mailto:kcrosby@cellsignal.com]
Sent: Friday, May 23, 2003 5:37 AM
To: Histonet
Subject: Cell Pellet Processing


Hi All,
Previously we were manually processing our cell pellets (with 
Histogel), with nice results.
We have switched to an auto-proccessor now, and have found that the 
pellets are shrinking and brittle.  We are using the same processing 
times and reagents as before.  We have seen similar shrinking with 
small pieces of mouse tissue.
Has anyone experienced this?  If you are successfully using an 
auto-processor for cell pellets, would you be willing to share your 
schedule?
Thanks in advance,
Katie


Katie Crosby
Manager, Immunohistochemistry
Cell Signaling Technology
166B Cummings Center
Beverly, MA  01915

Phone: 978-867-2352
Fax:  978-867-2402
E-mail:  kcrosby@cellsignal.com