RE: Breast Tissue

From:Tony Henwood

But don't tissues pass through alcohols during processing? Since tissues, these days, are rarely completely fixed in formalin prior to processing, then ethanol probably has a secondary fixing effect.
Just my thoughts,

Tony Henwood JP, BAppSc, GradDipSysAnalys, CT(ASC)
Laboratory Manager
The Children's Hospital at  Westmead,
Locked Bag 4001, Westmead, 2145, AUSTRALIA.
Tel: (02) 9845 3306
Fax: (02) 9845 3318

-----Original Message-----
From: Patsy Ruegg []
Sent: Saturday, 31 May 2003 2:50
Subject: RE: Breast Tissue

To all who hope to do her2neu expression on breast samples.
I attended a conference presented by a pathologist from Phenopath (lg. reference lab in Seattle (Dr. Gown)) who warned that her2neu expression was adversely effected by use of alcoholic fixatives.  Phenopath does a lot of her2neu tests and they are considered experts in this field.
-----Original Message-----
From: []
Sent: Thursday, May 29, 2003 2:28 PM
Subject: Breast Tissue

Does anyone out there in histoland have any suggestions for fatty breast tissue processing?  We are having a terrible time getting some of our breast tissue fixed well.    Thanks in advance for your responses.

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