RE: Breast Tissue

From:"Weems, Joyce"

Critical point!
 
We have a processor for fatty tissue and made this change in the final dehydration steps:
100% alcohol
Xylene
100% alcohol
Xylene
Xylene
Paraffin, etc.
 
This has helped tremendously. When tissues are cut too thick, we press between paper towels before embedding, which also has made quite a difference.
 
It is my understanding that 10% Formalin is the FDA approved precedure.
 
Happy weekend everyone! j
-----Original Message-----
From: Patsy Ruegg [mailto:pruegg@colobio.com]
Sent: Friday, May 30, 2003 12:50 PM
To: Histolady710@aol.com; HISTONET@pathology.swmed.edu
Subject: RE: Breast Tissue

To all who hope to do her2neu expression on breast samples.
I attended a conference presented by a pathologist from Phenopath (lg. reference lab in Seattle (Dr. Gown)) who warned that her2neu expression was adversely effected by use of alcoholic fixatives.  Phenopath does a lot of her2neu tests and they are considered experts in this field.
Patsy
-----Original Message-----
From: Histolady710@aol.com [mailto:Histolady710@aol.com]
Sent: Thursday, May 29, 2003 2:28 PM
To: HISTONET@pathology.swmed.edu
Subject: Breast Tissue

Does anyone out there in histoland have any suggestions for fatty breast tissue processing?  We are having a terrible time getting some of our breast tissue fixed well.    Thanks in advance for your responses.

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