RE: Breast Tissue
Critical point!
We
have a processor for fatty tissue and made this change in the final dehydration
steps:
100%
alcohol
Xylene
100%
alcohol
Xylene
Xylene
Paraffin, etc.
This
has helped tremendously. When tissues are cut too thick, we press between paper
towels before embedding, which also has made quite a difference.
It is
my understanding that 10% Formalin is the FDA approved
precedure.
Happy
weekend everyone! j
To
all who hope to do her2neu expression on breast samples.
I
attended a conference presented by a pathologist from Phenopath (lg. reference
lab in Seattle (Dr. Gown)) who warned that her2neu expression was adversely
effected by use of alcoholic fixatives. Phenopath does a lot of her2neu
tests and they are considered experts in this field.
Patsy
Does anyone out there in histoland have any
suggestions for fatty breast tissue processing? We are having a
terrible time getting some of our breast tissue fixed
well. Thanks in advance for your responses.
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