RE: Bovine tissue.

From:Patsy Ruegg

Mikael,
Using bovine proteins on bovine tissues should not be a problem as long as
it is a very good purified form with no contaminating proteins.  Most BSA I
see people use and have used myself is not purified well enough in my
experience.
Patsy

-----Original Message-----
From: Mikael Niku [mailto:mikael.niku@helsinki.fi]
Sent: Thursday, May 22, 2003 10:31 PM
To: Patsy Ruegg; Ian Montgomery; histonet@pathology.swmed.edu
Subject: Re: Bovine tissue.


Oh, I forgot to add: I'm using BSA as usual, and see no
problems. Why should this be a problem, adding some more
bovine protein(s) on bovine tissues?

Using (unpure) BSA with antibodies produced in cattle would be
a different matter.

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   Mikael Niku             URL: www.helsinki.fi/~mniku/
   University of Helsinki  Dept. Basic Veterinary Sciences
       - Mit#228#k#246# mielt#228# olen l#228#nsimaisesta sivistyksest#228#?
         Minusta se olisi erinomainen ajatus!
                                              - Gandhi
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----- Original Message -----
From: "Patsy Ruegg" 
To: "Ian Montgomery" ;

Sent: Friday, May 23, 2003 12:24 AM
Subject: RE: Bovine tissue.


> Ian, I would stay away from use of BSA which is often in antibody diluent
> buffers and use goat serum block and maybe some serum free protein block.
I
> like to block before the primary antibody and then again before the
> secondary if non-specific binding is anticipated.  I always do endogenous
> peroxidase block after the primary antibody in case there is some effect
on
> binding of the primary.
> Patsy






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