RE: Bovine tissue.
Ian, I
would stay away from use of BSA which is often in antibody diluent buffers and
use goat serum block and maybe some serum free protein block. I like to
block before the primary antibody and then again before the secondary if
non-specific binding is anticipated. I always do endogenous peroxidase
block after the primary antibody in case there is some effect on binding of the
primary.
Patsy
About
to use rabbit primaries with goat , fluorochrome tagged, anti-rabbit
secondaries on bovine tissues. Apart from the obvious blockers is there
anything else I should be thinking about with bovine tissue. At the moment the
tissue will be formalin fixed and paraffin embedded.
Ian.
Dr. Ian
Montgomery,
Histotechnology,
Graham Kerr Building,
Institute of
Biomedical & Life Sciences,
University of Glasgow,
Glasgow,
G12
8QQ.
Tel: 0141 339 8855
Office: 4652
Lab: 6644.
Pager: 07625
702883
e-mail: ian.montgomery@bio.gla.ac.uk
<< Previous Message | Next Message >>