RE: Bovine tissue.

From:Patsy Ruegg

Ian, I would stay away from use of BSA which is often in antibody diluent buffers and use goat serum block and maybe some serum free protein block.  I like to block before the primary antibody and then again before the secondary if non-specific binding is anticipated.  I always do endogenous peroxidase block after the primary antibody in case there is some effect on binding of the primary.
Patsy
-----Original Message-----
From: Ian Montgomery [mailto:ian.montgomery@bio.gla.ac.uk]
Sent: Thursday, May 22, 2003 8:56 AM
To: histonet@pathology.swmed.edu
Subject: Bovine tissue.

        About to use rabbit primaries with goat , fluorochrome tagged, anti-rabbit secondaries on bovine tissues. Apart from the obvious blockers is there anything else I should be thinking about with bovine tissue. At the moment the tissue will be formalin fixed and paraffin embedded.
Ian.

Dr. Ian Montgomery,
Histotechnology,
Graham Kerr Building,
Institute of Biomedical & Life Sciences,
University of Glasgow,
Glasgow,
G12 8QQ.
Tel: 0141 339 8855
Office: 4652
Lab: 6644.
Pager: 07625 702883
e-mail: ian.montgomery@bio.gla.ac.uk


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