RE: Air-drying cryosections
Hi Nidal,
No problem at all! Most antigens will survive this easily.
Chris van der Loos
Dept. of Cardiovascular Pathology
Academical Medical Center
Amsterdam - The Netherlands
Nidal Muvarak wrote:
>Date: 23 May 2003 12:00:49 -0500
>From: NIDAL E MUVARAK
>Subject: Air-drying cryosections
>
>Hi everyone. I have sectioned some lung and artery tissue (preserved in OCT)
>and left them air-dry at room temp for two days. This was not done on
purpose;
>our -80 freezer was being serviced and there wasn't another one anywhere
near.
>Anyway, after two days, I put the slides at -80. Does anyone think that this
>will cause any problems with my immunostaining, or even with other
>histological dyes (e.g. sirius red, VVG, etc)? Thanks.
>
>Nidal E Muvarak
>Associate Research Specialist
>Department of Biomedical Engineering
>University of Wisconsin-Madison
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