RE: Air-drying cryosections

From:Chris van der Loos

Hi Nidal,
No problem at all! Most antigens will survive this easily.
Chris van der Loos
Dept. of Cardiovascular Pathology
Academical Medical Center
Amsterdam - The Netherlands

Nidal Muvarak wrote:
 >Date: 23 May 2003 12:00:49 -0500
 >From: NIDAL E MUVARAK 
 >Subject: Air-drying cryosections
 >
 >Hi everyone. I have sectioned some lung and artery tissue (preserved in OCT)
 >and left them air-dry at room temp for two days. This was not done on 
purpose;
 >our -80 freezer was being serviced and there wasn't another one anywhere 
near.
 >Anyway, after two days, I put the slides at -80. Does anyone think that this
 >will cause any problems with my immunostaining, or even with other
 >histological dyes (e.g. sirius red, VVG, etc)? Thanks.
 >
 >Nidal E Muvarak
 >Associate Research Specialist
 >Department of Biomedical Engineering
 >University of Wisconsin-Madison




<< Previous Message | Next Message >>