Please can someone advise me on how to cut good cryosections of skin? I am 
having real trouble getting good mouse sections, the tissue tears and 
separates from the OCT as I'm cutting it. I've tried changing the 
temperature, from -20 to -15, as the manual for the cyrostat seem to be 
saying the temperature was too cold, changing the clearance angle, new 
blades, changing the speed - I'm exasperated. I know it does take a lot of 
practice to get the technique right, I learnt this with paraffins, but I 
really don't know what I'm doing wrong. Please help!! I have 200 samples to 
cut!!!
Yours desperately,
Nicola Cragg