Pre staining white biopsies for easy visibility in paraffin block s

From:Diana McCaig

I know this issue was addressed previously but would like some additional
assistance.  Years back I worked in a lab that colored their biopsies at the
grossing bench with an eosin solution.  I can not recall the strength or
concentration but for some reason a saturated alcoholic solution sounds
familiar.  I have tried this as well as adding 20 drops of 5% eosin/200 ml
to the first alcohol (75%) on our RMC processor.  But, neither method
colored the biopsies.  I do not want to use mercurichrome or methylene blue.
I want to use an eosin based solution.  Can anyone provide me with a recipe
of success.  I would prefer coloring them at the grossing bench rather than
in the processor but will try anything that will make the biopsies visible.
I don't like cutting by brail.
Diana McCaig, R.T. 
Charge Tech, Histology
Chatham Kent Health Alliance
519-352-6401 (3347)

	 
	 

	



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