Fwd: EM Staining questions

From:Ian Montgomery

Sara,
        Looking through the cannon balls how's the general fixation? Have you changed the fixation protocol or the final resin mixture? Uranyl acetate, is the solution fresh and how is it stored prior to use? Lead citrate, although it's said to mature, how old is the stain and again how is it stored? For your lead stains are you using fresh distilled water or my preference, freshly distilled and sonicated.
Ian.


Date: Wed, 21 May 2003 05:57:12 -0700 (PDT)
From: sara goldston <cogold13@yahoo.com>
Subject: EM Staining questions
To: histonet@pathology.swmed.edu

I work in electron microscopy. Lately, staining has been a problem. Sections have exhibiting poor contrast and/or considerable black deposits. In our lab, we utilize water-based and methylene-based uranyl acetate, Reynold's and Sato's lead citrate. In particular, the methylene UA


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Dr. Ian Montgomery,
Histotechnology,
Graham Kerr Building,
Institute of Biomedical & Life Sciences,
University of Glasgow,
Glasgow,
G12 8QQ.
Tel: 0141 339 8855
Office: 4652
Lab: 6644.
Pager: 07625 702883
e-mail: ian.montgomery@bio.gla.ac.uk


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