Morning all
I've been using Sigma's Naphthol AS-D chloroacetate esterase kit to 
stain arthritic mouse joints and surprisingly no neutrophils are 
staining up (although you can see they're there on H&E) but the mast 
cells are staining beautifully! I was wondering if the 
decalcification process (EDTA) affects the esterase activity of 
neutrophils but not mast cells? oes anyone have any ideas? It's not 
too much of a disaster, more a curiosity.
Cheers!
Joey
-- 
Dr Joey Shepherd
Postdoctoral scientist
Academic Unit of Genetics and Informatics
Division of Genomic Medicine
The University of Sheffield Medical School
M Floor, Royal Hallamshire Hospital
Sheffield S10 2JF
UK

Phone 0114 271-3007
Fax 0114-272-1104