Air-drying cryosections
Hi everyone. I have sectioned some lung and artery tissue (preserved in OCT) and left them air-dry at room temp for two days. This was not done on purpose; our -80 freezer was being serviced and there wasn't another one anywhere near. Anyway, after two days, I put the slides at -80. Does anyone think that this will cause any problems with my immunostaining, or even with other histological dyes (e.g. sirius red, VVG, etc)? Thanks.
Nidal E Muvarak
Associate Research Specialist
Department of Biomedical Engineering
University of Wisconsin-Madison
1550 Engineering Dr.; Rm. 2158
Madison, WI 53706-1609
Lab: (608) 265-8921; Office: (608) 265-4205;
Home: (608) 256-7934; Cell: (608) 332-6068
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