immunohistochemistry for GFP

From:d.gregg@juno.com

Dear Histonetters,
I will be starting a project looking for green flourescent protein
expressed by a virus in swine tissues. I will have to screen many organs
and don't look forward to doing it in a dark room by FA. Do any of you
have a protocol or Ab recommendation for an anti-GFP antibody that will
work well in paraffin sections (or frozen). I would prefer a good
monoclonal to a polyclonal. I have had background problems rather
consistently using rabbit polyclonal antibodies on swine tissues. Thanks
for any suggestions.

Douglas Gregg DVM PhD
Plum Island Animal Disease Center
Greenport, NY 11944
d.gregg@juno.com



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