Re: smudges

From:Jan Shivers

I have had that happen when ... a) the slide has not been completely
deparaffinized, and... b) when the tissue has not been adequately rinsed
with buffer or blown clear between steps (when using an automatic stainer,
especially on slides that have been proteinase-digested).

Jan Shivers
Scientist, IHC Lab
Univ. of Minnesota Veterinary Diagnostic Lab
1333 Gortner Ave.
St. Paul, MN  55108

----- Original Message -----
From: "Margaret Horne" 
Sent: Wednesday, May 14, 2003 12:26 PM
Subject: smudges

> Hi All ,  I am getting a smudge of AEC chromagen on some of my
> slides. It is on both tissue and slide indiscriminately. Sometimes
> pale , sometimes quite heavy,it even shows up in the deletion
> negatives.
>     Has anyone out there had the same trouble?
>       Thanks ,
>              Margaret
> Margaret Horne ,
> Histology Teaching Assistant,
> Dept. of B.SC.,
> Atlantic Veterinary College, U.P.E.I.,
> 550 University Ave., Charlottetown,
> P.E.I.,  C1A 4P3
> Canada

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