Re: immunohistochemistry for GFP

From:Gayle Callis

Chemicon has a mouse antiGFP - rabbit antiGFP. 

At 02:39 PM 5/6/2003 -0400, you wrote:
>Dear Histonetters,
>I will be starting a project looking for green flourescent protein
>expressed by a virus in swine tissues. I will have to screen many organs
>and don't look forward to doing it in a dark room by FA. Do any of you
>have a protocol or Ab recommendation for an anti-GFP antibody that will
>work well in paraffin sections (or frozen). I would prefer a good
>monoclonal to a polyclonal. I have had background problems rather
>consistently using rabbit polyclonal antibodies on swine tissues. Thanks
>for any suggestions.
>Douglas Gregg DVM PhD
>Plum Island Animal Disease Center
>Greenport, NY 11944
Gayle Callis
Research Histopathology Supervisor
Veterinary Molecular Biology - Marsh Lab
Montana State University - Bozeman
S. 19th and Lincoln St
Bozeman MT 59717-3610

406 994-6367 (lab with voice mail)
406 994-4303 (FAX)


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