Try adding levamisole to the chromagen
Rena Fail



 Any suggestions or methods to block normal occurring endogenous Alkaline
> Phosphatase in tissue during IHC staining.  
> 
> Note: I am not asking about blocking endogenous peroxidase or blocking
> endogenous biotin.
> Thanks in advance,
> 
> Carol Ann Bobrowitz
> Histology Laboratory
> Department of Physiology - Room 541
> Medical College of Wisconsin
> 8701 Watertown Plank Road
> Milwaukee, Wisconsin   53226
> 414-456-8179
> FAX 414-456-6546
>   
>