Hi Jim
We are using MMP-9, clone 56-2A4 (Oncogene), working conc. 1 ug Ig/ml after
HIER (microwave; 100 mM Tris - 5% urea, pH 9.5 - don't ask why, it works;
HIER in pressure cooker with citrate buffer will work also, though staining
is a little less intense). Couldn't get clone 15W2 to work properly on FFPE
material. No experience with MMP-3. Hope this helps.

Andi Kappeler
Institute of Pathology, Universtiy of Bern, Switzerland

----- Original Message -----
From: "jim" 
To: "Histonet" 
Sent: Tuesday, May 13, 2003 4:54 AM
Subject: MMP-3 and MMP-9


> Has anyone any optimisation details (controls and pretreatments) for MMP-3
> and MMP-9 on FFPE material. Thanks in advance.
>
> Jim Manavis
> Senior Hospital Scientist
> Department of Neuropathology
> Institute of Medical & Veterinary Science
> Adelaide, SA, 5000
> Australia
> Phone: 61-08-8222-3668
> FAX: 61-08-8222 3204
> e.mail: jim.manavis@imvs.sa.gov.au
> Disclaimer: Not this little black duck!
>
>