Re: Lobster claws.
First, a question: what are you after? Can you dissect away the
exoskeleton to get at your subject material?
If not, I'd try decalcifiying with EDTA (crustaceans have calcite
rods in their cuticles), and possibly treatment with a chitinase. For
which I don't have a recipe -- my references to my past life as a
crustacean biologist are packed away.
Exoskeletons of small crustaceans can be sectioned with glass and
diamond knives, but given the size of lobster claws ... you're not
sectioning the whole thing are you?
Lobster claws and don't start all the various methods of
preparation that have the final destination of the human stomach.
Although that's the method of my first choice. Been fixed in
Davidson's so any hints and tips on the processing that won't leave
me with a crunchy, knife blunting exoskeleton.
Dr. Ian Montgomery,
Graham Kerr Building,
Institute of Biomedical & Life Sciences,
University of Glasgow,
Tel: 0141 339 8855
Pager: 07625 702883
Supervisor, BBPIC microscopy facility
Department of Animal Sciences
University of Wisconsin
1675 Observatory Drive
Madison, WI 53706 - 1284
voice: (608) 263-4162
fax: (608) 262-5157 (dept. fax)
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