Re: Calretinin

From:Richard Cartun

Are you using a polyclonal or a monoclonal antibody?

Richard Cartun

>>> Cindy DuBois  05/12/03 06:06PM >>>

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From: Cindy/Rick DuBois 
Date: Wed, 7 May 2003 09:58:50 -0700 (PDT)
To: ccdub@earthlink.net 
Subject: Calretinin

Our docs have noticed that our Calretinin does not stain on cell
blocks.
These specimens are initially received in cytolyte (alcohol based
fixative),
a cell block is prepared and processed in the tissue processor with the
rest
of the tissue.  
The control we are using stains positively and is a mesothelioma from
the
lung (formalin fixed tissue).
Any suggestions on how to get the cell blocks to stain?  Any help would
be
greatly appreciated.
Cindy DuBois HT, ASCP
Delta Pathology Assoc.
Stockton, CA





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