Re: Achrived tissue viability when stored in 10% formalin
Lee Luna's "AFIP manual" (1968) has several suggestions for restoring
basophilia to 'overfixed' specimens, which is what I think you were asking
about..
1. Bring paraffin sections to water.
2. Place sections in one of the following:
5% aqueous sodium bicarbonate, 4 hours to overnight.
OR
5% aqueous ammonium sulphide overnight
OR
5% aqueous periodic acid overnight
3. Wash in tap water
4. Stain as desired.
As for "viable" tissue coming out of formalin, I know several well-educated
people who firmly believe in the re-animation of dead tissue, so that may
actually be the question!
LMHAMILTON1@aol.com wrote:
Hello,
The company I work for has been asked by one of our clients how long tissue
(animal) can be stored in formalin and still be viable. We have gone back
to tissue stored for ~10yrs. but the staining was poor. If there is any
advice on where we can find documentation of this information it would be
appreciated. Any thoughts on the time frame also are welcome.
Thank You,
Loriann
Geoff
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Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
675 Hoes Lane, Piscataway, NJ 08854
voice: (732)-235-4583; fax: -4029
mcauliff@umdnj.edu
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