RE: muscle snap freezing artifact

From:

Rena Fail

Message

Jeremy,

place the frozen muscle in cold saline 2-8 degrees for approximately 30 minutes. It is important to blot the specimen thoroughly after removing from the saline. Refreeze. This method eliminates most of the ice crystal artifact.

Rena Fail

Medical University of SC
Charleston,SC

USA

-----Original Message-----
From: Jeremy Browne [mailto:j.browne@auckland.ac.nz]
Sent: Monday, May 05, 2003 7:48 PM
To: histonet@pathology.swmed.edu
Subject: muscle snap freezing artifact

Anyone out there with advice or key tips for eliminating ice crystal artifact in snap frozen muscle samples?

Thanks

Jeremy Browne

Liggins Institute

University of Auckland

NZ (09) 373 7599 extn 86444

j.browne@auckland.ac.nz

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