Sigma has Trizma pH 7.4 Pre-set crystals.  Thats what we switched to when we
ran out of their buffer.  The cat.# is T 7693 and it comes in two sizes.  A
100g goes a long way.  Good luck.
 
Juan C. Gutierrez, HT(ASCP) 
Histology Supervisor 
Christus Santa Rosa Healthcare 
(210)704-2533 
Juan_Gutierrez@srhc.iwhs.org 

-----Original Message-----
From: WWmn916@aol.com [mailto:WWmn916@aol.com]
Sent: Sunday, May 11, 2003 10:26 PM
To: histonet@pathology.swmed.edu
Subject: Tris or Phosphate buffer?


Sigma is no longer making premade Trisma Buffer pH 7.4 
We have always used their product in making our solution for the NADH muscle
stain.   
I've experimented with a Sodium Phosphate diabasic/monobasic solution and
the results looked the same as a NADH muscle stained with a Trisma buffer
component. 

Can anyone tell me what the difference between the two buffers are and what
factors I need to consider, if any, in transitioning to making the sodium
phosphate buffer to replace the Trisma buffer? 

Lastly, does anyone know of a different supplier of premadeTrisma buffers,
other than Sigma or Fisher (since Fisher gets their tris buffer from Sigma?)


Thanks, 
Deb King HT 
Sacramento, CA