RE: SV40 Help

From:"Morken, Tim"

Lee, We use SV40(pb416) from Oncogene Research in the US
). We use FFPE tissue, steam antigen retrieval for 20 minutes in citrate
buffer (BioGenex Citra) and then use an antibody dilution of 1:500. we
detect with the dako LSAB2 alk phos kit and fast red chromagen. As for
contols, we do have some, but we don't share them. Sorry!

Tim Morken
Centers for Disease Control
Atlanta, GA

-----Original Message-----
From: LD Ridley [] 
Sent: Monday, May 12, 2003 8:54 AM
Subject: SV40 Help

Hi everyone,
             Does anyone have any experience of working with the SV40
(pab101) antibody on FFPE SV40 infected tissue.I would particularly like to
know what method of antigen retrieval you used to get successful results and
where you were able to obtain a SV40 positive tissue block from. Thanks in
advance for your help

Lee Ridley

Birmingham Women's Hospital
Reproductive & Child Health
Academic Unit 3RD Floor
United Kingdom
B15 2TG

Tel: +44 (0)121 6074754

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