RE: Embedding Hydrophilic Polymer
-----Original Message-----
From: HistoNet Server [mailto:histonet@pathology.swmed.edu]
Sent: Friday, May 02, 2003 1:03 AM
To: HistoNet Server
Subject: Daily Digest
----------------------------------------------------------------------
Date: 1 May 2003 00:05:07 -0500
From: "Williams, Blanche"
Subject: Out of Office AutoReply: Daily Digest
I will be out of the office May 1st and May 2nd .. If you have a question
regarding histology please contact Diane Coulter at 47196. If you have an
administrative question please contact Marilyn McAllister at 48516
----------------------------------------------------------------------
Date: 1 May 2003 04:30:32 -0500
From: Gordon Couger
Subject: Re: OT - Spam
There are a lot of good spam fillers out there but you don't want to
automatically delete messages. sort the spam into a folder. All the spam
filters get some real email with the spam and some sam with the mail. I get
over 200 a day and they are not hard to manage with a good spam filter and
sorting the meal to folders.
Keep your work address as free as you can from news groups and such where
spam bots can harvest it> Get a second account to in fact wiht the non
working world. You will have less problems at work and less aggravation all
around.
The government, big ISPs and some other stake holder are going try to patch
things up to reduce the problem but the way email works it can't be fixed
and be backward comp with old email systems.
Building a much more secure email system is quite possible one you get
everyone to agree on the method, format and such. Well I guess it isn't
possible after all
Gordon
Gordon Couger
Stillwater, OK
www.couger.com/gcouger
net>
To: "histonet"
Sent: Wednesday, April 30, 2003 9:46 AM
Subject: Re: OT - Spam
: on 29/04/2003 8:21 AM, marjorie lehman at Marjorie.Lehman@unilever.com
: wrote:
:
: >
: > It would appear someone got hold of the Histonet list.
: > I started getting those Urgent Request things and delete anything that
looks
: > like one now.
: > Then I started getting other ads, including ways to "improve" certain
: > anatomical features I don't have!! Why anyone would send those to a
person
: > named Marjorie is beyond me!!
: > The final straw was when I started getting offers for videos entitled
"Girls,
: > Girls, Girls" etc
: > I contacted my IT people and they told me they are working on it.
(Standard
: > Answer). Their advice is to not open them if you can recognize what they
are &
: > never, ever answer them -even the ones which say click here if you don't
want
: > us to send any more.
: > Marjorie - a person of the female persuasion
: >
: >
: If you are using Outlook Express, you can use the Rules feature to send
such
: things directly to the delete file, without you having to see them. It
: doesn't stop them arriving, but you don't have to know about it. I expect
: other mail programmes have a similar feature. And it is very important not
: to answer - doing so confirms for the spammer that your e-mail address is
: valid, and you'll be deluged with the stuff.
:
: Lesley Weston.
:
:
:
:
----------------------------------------------------------------------
Date: 1 May 2003 07:46:09 -0500
From: "Sebree Linda A."
Subject: Neu N
Good Morning,
Our neuropathologist would like to have the antibody Neu N on board. Anyone
out there using it? From where? Vendors welcome to reply. We have Ventana
instruments so if anyone is using Neu N on VMS stainers, I'd especially like
to hear from you.
Thanks,
Linda
Linda A. Sebree, HT(ASCP)
University of Wisconsin Hospital & Clinics
IHC/ISH Laboratory
A4/204-2472
600 Highland Ave.
Madison, WI 53792
(608)265-6596
FAX: (608)262-7174
----------------------------------------------------------------------
Date: 1 May 2003 07:49:47 -0500
From: Hermina Borgerink
Subject: RE: active caspase 3 antibody
Cell Signaling Technology, rabbit polyclonal antibody which works well in
both
mouse and non-human primate tissue. Cat. #9661S for 100 microliters
($250.00) and #9661L for 300 microliters ($600.00). A 1:50 dilution works
well for me.
Hermina
Hermina M. Borgerink, BA, HTL(ASCP)IHQ
Wake Forest University School of Medicine
Department of Pathology
Winston-Salem, NC
(336) 716-1538
- -----Original Message-----
From: Nancy Lemke [mailto:nperson211@comcast.net]
Sent: Wednesday, April 30, 2003 5:12 PM
To: histonet@pathology.swmed.edu
Subject: active caspase 3 antibody
Histonetters!
Does anyone have a suggestion for an antibody for activated caspase 3
that will work in FFPE tissues?
Thanks in advance,
Nancy Lemke
Hermelin Brain Tumor Center
Henry Ford Hospital
Detroit, MI
----------------------------------------------------------------------
Date: 1 May 2003 08:15:18 -0500
From: Noreen Gilman
Subject: Re: NSH Virtual Library
Vinnie, I heard from the state of Fla, as well as from NSH. They accept
ceu's from both ASCP & NSH. Thanks to you and to all others who
responded.
Gratefully,
Noreen
Noreen Gilman, BS, HT (ASCP) QIHC
Histopathology Supervisor
Broward General Medical Center
Ft. Lauderdale, FL 33316
954.355.4358 Phone
954.355.4139 Fax
954.387.0213 Pager
>>> Vinnie Della Speranza 04/30/03 11:45AM >>>
Noreen,
I would urge you to consult with someone knowledgeable about the
licensing requirements in your state. I know that Florida requires a
specified number of continuing education credits but I do not know if
they require that they be in a classroom setting where the participant
can ask questions. My instincts tell me that the virtual library will
be
of benefit to you but it's best to check first.
Vinnie Della Speranza
NSH Vice President
>>> Noreen Gilman 04/30/03 07:28AM >>>
Can I use these credits (and any other CME/CE) offered by NSH & CAP,
to
renew my Florida license?
Thanks
Noreen
Noreen Gilman, BS,HT(ASCP)QIHC
Histopathology Supervisor
Broward General Medical Center
Ft. Lauderdale, FL 33316
954.355.4358 Phone
954.355.4139 Fax
954.387.0213 Pager
>>> 04/29/03 09:14PM >>>
The first three courses in the NSH Virtual Library are now available.
What
a great way to get some continuing education credits in the comfort of
your
own home. To find out more, just log on to the NSH web site at
www.nsh.org.
Freida Carson
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Vinnie, I heard from the state of Fla, as well as from NSH. They accept
ceu's from both ASCP & NSH. Thanks to you and to all others who
responded.
Gratefully,
Noreen
Noreen Gilman, BS, HT (ASCP) QIHC
Histopathology
Supervisor
Broward
General Medical Center
Ft. Lauderdale, FL 33316
954.355.4358
Phone
954.355.4139 Fax
954.387.0213 Pager
>>>
Vinnie
Della Speranza <dellav@musc.edu> 04/30/03 11:45AM
>>>
Noreen,
I would urge you to consult with someone
knowledgeable about the
licensing requirements in your state. I know that
Florida requires a
specified number of continuing education credits but I
do
not know if
they require that they be in a classroom setting where the
participant
can ask questions. My instincts tell me that the virtual
library
will be
of benefit to you but it's best to check first.
Vinnie Della
Speranza
NSH Vice President
>>> Noreen Gilman
<ngilman@nbhd.org> 04/30/03 07:28AM >>>
Can I use these
credits (and any other CME/CE) offered by NSH & CAP,
to
renew my
Florida license?
Thanks
Noreen
Noreen Gilman,
BS,HT(ASCP)QIHC
Histopathology Supervisor
Broward General Medical
Center
Ft. Lauderdale, FL 33316
954.355.4358 Phone
954.355.4139
Fax
954.387.0213 Pager
>>> <FreidaC@aol.com>
04/29/03 09:14PM >>>
The first three courses in the NSH Virtual
Library are now available.
What
a great way to get some
continuing
education credits in the comfort of
your
own home. To find out
more, just log on to the NSH web site at
www.nsh.org.Freida
Carson
I would like to visualize with SEM the pores that we have created in a
hydrophilic polymer by looking at the cross-section. The hydrophilic
polymer is coated on stainless steel. Is there an embedding polymer
and
a process that someone is familiar with?
Jack Frautschi
University of Texas at Dallas
903-675-8081
903-681-2565 (Cell)
903-670-1205 (Fax)
- --Boundary_(ID_jzeibxKZwp5Q2iO8ZWoIZQ)--
----------------------------------------------------------------------
Date: 1 May 2003 09:15:47 -0500
From: Millicent Pope
Subject: More on blue smooth muscle
Thank you Vinnie and Tere for taking the time to think about my staining
problem. I wish I could submit a photo of one of my slides so you could
see what I am talking about! All I can say in hopes of clarification is
the blue spots are light blue blobs, with the normal or a slightly lower
number of dark nuclei in them. It's not a large number of nuclei
clustered together making the tissue blue. It's just light blue
cytoplasm. And the slides I've made have all had the blue spots in the
same places.
Thanks again-
Milli Pope
Texas A&M University
VAPH Histology
----------------------------------------------------------------------
Date: 1 May 2003 09:45:29 -0500
From: TJasper@smdc.org
Subject: Knife Sharpening
My thanks to all for the knife sharpening information.
Thomas Jasper HT(ASCP) BAS
Anatomic Pathology Coordinator
SMDC Clinical Laboratory
Duluth, MN
tjasper@smdc.org
----------------------------------------------------------------------
Date: 1 May 2003 09:49:09 -0500
From: "Kalina, Marion"
Subject: Embedding Hyrophilic Polymers
Date: 28 Apr 2003 17:00:30 -0500
From: Frautschij@aol.com
Subject: Embedding Hydrophilic Polymers
Greetings,
I would like to visualize with SEM the pores that we have created in a
hydrophilic polymer by looking at the cross-section. The hydrophilic
polymer
is coated on stainless steel. Is there an embedding polymer and a process
that someone is familiar with?
Jack Frautschi, Ph.D.
University of Texas at Dallas
903-675-8081
903-681-2565 (Cell)
903-670-1205 (Fax)
Hi Jack,
You might want to look into Nanoplast embedding media. Have a look at this
web page for more information.
www.polysciences.com/shop/datasheet.asp
Marion Kalina
marion.kalina@hrcc.on.ca
This information is directed in confidence solely to the person named above
and may not otherwise be distributed, copied or disclosed. Therefore, this
information should be considered strictly confidential. If you have received
this email in error, please notify the sender immediately via a return email
for further direction. Thank you for your assistance.
----------------------------------------------------------------------
Date: 1 May 2003 10:16:10 -0500
From: Lesley Weston
Subject: Re: OT - Spam
You could do this, but if you use the Rules feature rather than the Spam
Filter feature, you can tell it which senders or recipients or subjects to
delete individually. It's a small nuisance, but there is some satisfaction
in consigning each spammer to the outer darkness as they pop up. When I
download my mail these days, my Deleted Items folder fills up at twice the
rate of the Inbox folder.
Lesley Weston.
on 01/05/2003 2:22 AM, Gordon Couger at gcouger@provalue.net wrote:
> There are a lot of good spam fillers out there but you don't want to
> automatically delete messages. sort the spam into a folder. All the spam
> filters get some real email with the spam and some sam with the mail. I
get
> over 200 a day and they are not hard to manage with a good spam filter and
> sorting the meal to folders.
>
> Keep your work address as free as you can from news groups and such where
> spam bots can harvest it> Get a second account to in fact wiht the non
> working world. You will have less problems at work and less aggravation
all
> around.
>
> The government, big ISPs and some other stake holder are going try to
patch
> things up to reduce the problem but the way email works it can't be fixed
> and be backward comp with old email systems.
>
> Building a much more secure email system is quite possible one you get
> everyone to agree on the method, format and such. Well I guess it isn't
> possible after all
>
> Gordon
>
> Gordon Couger
> Stillwater, OK
> www.couger.com/gcouger
>
> net>
> To: "histonet"
> Sent: Wednesday, April 30, 2003 9:46 AM
> Subject: Re: OT - Spam
>
>
> : on 29/04/2003 8:21 AM, marjorie lehman at Marjorie.Lehman@unilever.com
> : wrote:
> :
> : >
> : > It would appear someone got hold of the Histonet list.
> : > I started getting those Urgent Request things and delete anything that
> looks
> : > like one now.
> : > Then I started getting other ads, including ways to "improve" certain
> : > anatomical features I don't have!! Why anyone would send those to a
> person
> : > named Marjorie is beyond me!!
> : > The final straw was when I started getting offers for videos entitled
> "Girls,
> : > Girls, Girls" etc
> : > I contacted my IT people and they told me they are working on it.
> (Standard
> : > Answer). Their advice is to not open them if you can recognize what
they
> are &
> : > never, ever answer them -even the ones which say click here if you
don't
> want
> : > us to send any more.
> : > Marjorie - a person of the female persuasion
> : >
> : >
> : If you are using Outlook Express, you can use the Rules feature to send
> such
> : things directly to the delete file, without you having to see them. It
> : doesn't stop them arriving, but you don't have to know about it. I
expect
> : other mail programmes have a similar feature. And it is very important
not
> : to answer - doing so confirms for the spammer that your e-mail address
is
> : valid, and you'll be deluged with the stuff.
> :
> : Lesley Weston.
> :
> :
> :
> :
>
----------------------------------------------------------------------
Date: 1 May 2003 10:16:25 -0500
From: marjorie lehman
Subject: Fluorescence Micrography
Many many thanks to those who responded to my question. Somewhere, in my
(rapidly failing) mind, I thought there was such a thing as a broad band
filter. So now we're going to get Leica to give us an estimate and tell The
Boss we need it for those people who just don't want to manipulate photos.
Thanks again, once again Histonet has saved my bacon.
Marge
----------------------------------------------------------------------
Date: 1 May 2003 10:30:57 -0500
From: "Dawson, Glen"
Subject: RE: FW: URGENT RESPONSES NEEDED, email fraud
I keep a folder for these. If I count the ones I've deleted, I've received
more than 70 scam emails like this one. I attempted to respond to one with
a nasty email and it came back as undeliverable.
Glen D.
----------------------------------------------------------------------
Date: 1 May 2003 11:00:49 -0500
From: Roberta Horner
Subject: Cryocut 1800 parts
Does anyone have an old Reich-Jung Cryocut 1800? The glass lid of ours
has cracked and we would like to replace the lid. If anyone has a lid
or know where we can get a replacement I would appreciate it if you
would let me know.
Roberta Horner HT HTL
Animal Diagnostic Lab
Penn State University
******************* NOTE *******************
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contained in the following MIME Information.
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Does anyone have an old Reich-Jung Cryocut 1800?
The
glass
lid of ours has cracked and we would like to replace the lid. If
anyone
has a
lid or know where we can get a replacement I would appreciate it if you
would
let me know.
Roberta Horner HT HTL
Animal Diagnostic Lab
Penn State University
- --Boundary_(ID_u1MyBlEc6WGtOWCH7gYWKg)--
----------------------------------------------------------------------
Date: 1 May 2003 11:16:00 -0500
From: "Chung, Luong"
Subject: IHC control slides/blocks
Hello all,
Can anyone tell me a proper way to store the control slides or blocks for
IHC? Can I cut a bunch of slides and store in a box?
or cut the block as needed? How long should a control block or slides be
kept? I have some control blocks work 6 months ago
and now it's not working. Any advise should be helpful.
Thanks
Bruce Chung, AP manager
Pheobe Putney Mem. Hosp.
HIPAA Privacy Rule requires covered entities to safeguard certain Protected
Health Information (PHI) related to a person's healthcare. Information being
sent to you may include PHI, after appropriate consent, acknowledgement, or
authorization from the patient or under circumstances that do not require
patient authorization. You, the recipient, are obligated to maintain PHI in
a safe and secure manner. You may not re-disclose this patient information
without additional patient consent or as required by law. Unauthorized
re-disclosure or failure to safeguard PHI could subject us, or you, to
penalties described in federal (HIPAA) and state law. If you, the reader of
this message, are not the intended recipient, or the employee or agent
responsible to deliver it to the intended recipient, please notify us
immediately and destroy the related message. Thanks for your help.
----------------------------------------------------------------------
Date: 1 May 2003 11:19:45 -0500
From: ALLISON@CARDIFF.AC.UK
Subject: A UK perspective
Go easy before you boast about your e-mail cicular messages.
It is two weeks since I was able to state that every living being had
writen to me!Russ Allison,
Dental School
Cardiff
Wales
----------------------------------------------------------------------
Date: 1 May 2003 11:45:59 -0500
From: Anita Westbrook
Subject: Decal Solutions
Since we are on the subject of decalcification, I was wondering what brand
of decal solutions everyone is using?
Thanks! Anita
This email and any files transmitted with it are confidential and intended
solely for the use of the individual or entity to whom they are addressed.
If you have received this email in error please contact the system
administrator for Southeast Missouri Hospital at infosys@sehosp.org.
----------------------------------------------------------------------
Date: 1 May 2003 11:46:18 -0500
From: "Gutierrez, Juan"
Subject: RE: IHC control slides/blocks
Refrigerate your blocks, and if you cut sections ahead of time, freeze them
at -80c.
Cutting through the block ahead of time is recomended. That way you can
test your sections from beginning to end to make sure the antigen is not
lost.
Hope this helps,
Juan C. Gutierrez, HT(ASCP)
Histology Supervisor
Christus Santa Rosa Healthcare
(210)704-2533
Juan_Gutierrez@srhc.iwhs.org
- -----Original Message-----
From: Chung, Luong [mailto:lchung@ppmh.org]
Sent: Thursday, May 01, 2003 11:11 AM
To: 'histonet@pathology.swmed.edu'
Subject: IHC control slides/blocks
Hello all,
Can anyone tell me a proper way to store the control slides or blocks for
IHC? Can I cut a bunch of slides and store in a box?
or cut the block as needed? How long should a control block or slides be
kept? I have some control blocks work 6 months ago
and now it's not working. Any advise should be helpful.
Thanks
Bruce Chung, AP manager
Pheobe Putney Mem. Hosp.
HIPAA Privacy Rule requires covered entities to safeguard certain Protected
Health Information (PHI) related to a person's healthcare. Information being
sent to you may include PHI, after appropriate consent, acknowledgement, or
authorization from the patient or under circumstances that do not require
patient authorization. You, the recipient, are obligated to maintain PHI in
a safe and secure manner. You may not re-disclose this patient information
without additional patient consent or as required by law. Unauthorized
re-disclosure or failure to safeguard PHI could subject us, or you, to
penalties described in federal (HIPAA) and state law. If you, the reader of
this message, are not the intended recipient, or the employee or agent
responsible to deliver it to the intended recipient, please notify us
immediately and destroy the related message. Thanks for your help.
----------------------------------------------------------------------
Date: 1 May 2003 11:46:37 -0500
From: Luis Chiriboga
Subject: RE: IHC control slides/blocks
recommended reading the article by ken Wester et al, Applied
Immunohistochemistry 8(1) 61-70, 2000
- -----Original Message-----
From: Chung, Luong [mailto:lchung@ppmh.org]
Sent: Thursday, May 01, 2003 12:11 PM
To: 'histonet@pathology.swmed.edu'
Subject: IHC control slides/blocks
Hello all,
Can anyone tell me a proper way to store the control slides or blocks for
IHC? Can I cut a bunch of slides and store in a box?
or cut the block as needed? How long should a control block or slides be
kept? I have some control blocks work 6 months ago
and now it's not working. Any advise should be helpful.
Thanks
Bruce Chung, AP manager
Pheobe Putney Mem. Hosp.
HIPAA Privacy Rule requires covered entities to safeguard certain Protected
Health Information (PHI) related to a person's healthcare. Information being
sent to you may include PHI, after appropriate consent, acknowledgement, or
authorization from the patient or under circumstances that do not require
patient authorization. You, the recipient, are obligated to maintain PHI in
a safe and secure manner. You may not re-disclose this patient information
without additional patient consent or as required by law. Unauthorized
re-disclosure or failure to safeguard PHI could subject us, or you, to
penalties described in federal (HIPAA) and state law. If you, the reader of
this message, are not the intended recipient, or the employee or agent
responsible to deliver it to the intended recipient, please notify us
immediately and destroy the related message. Thanks for your help.
----------------------------------------------------------------------
Date: 1 May 2003 12:30:41 -0500
From: Bernard Ian R SSgt 59 CRES/MSROP
Subject: RE: Decal Solutions
I use an excellent brand by StatLab Medical Products called "Decal
Plus"(Formic acid based).
32 oz for $15.00; Catalog number SL85-32.
Order Tel number: 1-800-442-3573
Thanks
SSgt Bernard
- -----Original Message-----
From: Anita Westbrook [mailto:awestbr@sehosp.org]
Sent: Thursday, May 01, 2003 11:39 AM
To: histonet
Subject: Decal Solutions
Since we are on the subject of decalcification, I was wondering what brand
of decal solutions everyone is using?
Thanks! Anita
This email and any files transmitted with it are confidential and intended
solely for the use of the individual or entity to whom they are addressed.
If you have received this email in error please contact the system
administrator for Southeast Missouri Hospital at infosys@sehosp.org.
----------------------------------------------------------------------
Date: 1 May 2003 13:00:56 -0500
From: mari.ann.mailhiot@leica-microsystems.com
Subject: Re: Cryocut 1800 parts
Hi Roberta
Your part number for the glass lid is 14041618491. If you need further
assistance please cintact me here at Leica.
Regards
Mari Ann Mailhiot BA HT ASCP
Application Specialist
Leica Technical Assistance Center
800 248 0123 x7267
847 236 3063 fax
mari.ann.mailhiot@leica-microsystems.com
www.leica-microsystems.com
Roberta Horner
To: Histonet
cc:
05/01/2003 10:46 Subject: Cryocut 1800 parts
AM
Does anyone have an old Reich-Jung Cryocut 1800? The glass lid of ours has
cracked and we would like to replace the lid. If anyone has a lid or know
where we can get a replacement I would appreciate it if you would let me
know.
Roberta HornerHT HTL
Animal Diagnostic Lab
PennStateUniversity
----------------------------------------------------------------------
Date: 1 May 2003 13:01:15 -0500
From: Bernard Ian R SSgt 59 CRES/MSROP
Subject: LOOKING FOR GOOD SORCE FOR THESE 2 CHEMICAL PRODUCTS.
Looking for a good source for Gold Chloride in the GMS(already made or dry
if possible). and Schiff Reagent for PAS stain
Also, Paraosaniline used in Gridley's Fungus stain.
Thanks
Pray today as its our National Day of Prayer
God Bless you all
Bernie
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Looking for
a good source for Gold Chloride in the GMS(already made or dry if
possible). and Schiff Reagent for PAS stain
Also,
Paraosaniline
used in Gridley's Fungus stain.
Thanks
Pray today as
its
our National Day of Prayer
God Bless you
all
Bernie
- --Boundary_(ID_LTuUhrtXWgkO8PIXMIVXLg)--
----------------------------------------------------------------------
Date: 1 May 2003 13:30:13 -0500
From: Elizabeth Chlipala
Subject: RE: Decal Solutions
Anita
We routinely make up our own decal of 5% formic acid. We find this to
be the most cost effective way to decal. It works great and it does not
cost very much.
Liz
Elizabeth Chlipala, BS, HTL(ASCP)
Premier Histology Laboratory, LLC
P.O. Box 18592
Boulder, Colorado 80308
Office (303) 735-5001
Fax (303) 735-3540
lizchlipala@premierhistology.com
www.premierhistology.com
- -----Original Message-----
From: Anita Westbrook [mailto:awestbr@sehosp.org]
Sent: Thursday, May 01, 2003 9:39 AM
To: histonet
Subject: Decal Solutions
Since we are on the subject of decalcification, I was wondering what
brand
of decal solutions everyone is using?
Thanks! Anita
This email and any files transmitted with it are confidential and
intended
solely for the use of the individual or entity to whom they are
addressed.
If you have received this email in error please contact the system
administrator for Southeast Missouri Hospital at infosys@sehosp.org.
----------------------------------------------------------------------
Date: 1 May 2003 13:45:56 -0500
From: Gayle Callis
Subject: Total quenching of peroxidase and pseudoperoxidases, in RBC's
If RBC's and other endogenous peroxidases/pseudoperoxidases are a problem,
the glucose oxidase method is far superior to take them out. It works on
acetone and/or paraffin sections, although I have not used it on the
latter, that was passed on from Carrie Kyle Byrne. It is wonderful for not
chewing up frozen sections and really cleans up a lot of diffuse background
plus specifics. It was originally used for IHC on Eosinophils in frozen
sections.
GLUCOSE OXIDASE BLOCK FOR ENDOGENOUS PEROXIDASE/PSEUDOPEORXIDASES IN
FROZEN/PARAFFIN SECTIONS
Reference: Andrew SM, Jasani B. An improved method for the inhibition of
endogenous peroxidase non-deleterious to lymphocyte surface markers.
Application to immunoperoxidase studies on eosinophil-rich tissue
preparations. Histochemical Journal 19:426-30, 1987
All forms of endogenous peroxidase may not be inhibited by the usual
methanol/hydrogen peroxide or buffer/hydrogen peroxide blocking mixtures.
This method produces nascent hydrogen peroxide that is preferable to the
normal methods using preformed hydrogen peroxide (which you add or buy in
your endogenous peroxide blocker). This new method to block 'peroxidatic
activity' is consistently complete. This method is an enzymatic reaction
that produces a slow, steady, very low concentration of hydrogen peroxide.
It is particularly useful for monoclonal antibody staining on frozen
sections that are minimally fixed with acetone.
Procedure:
Glucose (Sigma G 5250) 0.180 g
Glucose oxidase (Sigma G 6641) 0.005 g
Sodium azide 0.0065g
DPBS 50 ml
This procedure has doubled concentration of all reagents used in original
reference.
Blocking Protocol
1. Incubate sections 1 hour at 37#161#C waterbath. You do no have to
prewarm
buffer mixed with reagents before immersing slides.
2. Rinse in DPBS 3 changes/5 minutes each
3. Proceed with immunostaining
Preweigh and freeze down aliquots of glucose oxidase calculated for 100 mls
working buffer, bring out, and dissolve in DPBS/sodium azide buffer,
immerse slides, incubate, rinse, etc.
We make up large quantity of DPBS with azide to save time. Sodium azide is
toxic to you, safer this way. Keeps for months! We labet this Glucose
Oxidase Buffer.
Gayle Callis
MT,HT,HTL(ASCP)
Research Histopathology Supervisor
Veterinary Molecular Biology - Marsh Lab
Montana State University - Bozeman
S. 19th and Lincoln St
Bozeman MT 59717-3610
406 994-6367 (lab with voice mail)
406 994-4303 (FAX)
email: gcallis@montana.edu
----------------------------------------------------------------------
Date: 1 May 2003 13:46:15 -0500
From: Gayle Callis
Subject: Decalcifying solutions
WE always made up our own reagents to fit the project, but in a busy
clinical laboratory, you may want to have both a buffered formic acid
solution and rapid HCL type solution. There are times when you need the
gentler Formic acid and same for more rapid decalcifying with HCl.
There are many good sources of commercial decalcifiers - just check for
types of acids.
Using decalcifiers can be done creatively, in particular, there is no law
to say you can't dilute a strong HCl solution from 12 -15% HCl content
(MSDS) down to 6 - 7.5%. Formic acid solutions are already dilute,
generally around 4 - 5% if you calculate the molarity compared to % in MSDS.
Gayle Callis
MT,HT,HTL(ASCP)
Research Histopathology Supervisor
Veterinary Molecular Biology - Marsh Lab
Montana State University - Bozeman
S. 19th and Lincoln St
Bozeman MT 59717-3610
406 994-6367 (lab with voice mail)
406 994-4303 (FAX)
email: gcallis@montana.edu
----------------------------------------------------------------------
Date: 1 May 2003 14:16:10 -0500
From: MDiCarlo@KaleidaHealth.Org
Subject: RE: Decal Solutions
I agree with Beth. I also make my own decal except I use 10% formic acid.
Peggy DiCarlo HT(ASCP)
- -----Original Message-----
From: Elizabeth Chlipala [mailto:lizchlipala@premierhistology.com]
Sent: Thursday, May 01, 2003 14:20
To: 'Anita Westbrook'; 'histonet'
Subject: RE: Decal Solutions
Anita
We routinely make up our own decal of 5% formic acid. We find this to
be the most cost effective way to decal. It works great and it does not
cost very much.
Liz
Elizabeth Chlipala, BS, HTL(ASCP)
Premier Histology Laboratory, LLC
P.O. Box 18592
Boulder, Colorado 80308
Office (303) 735-5001
Fax (303) 735-3540
lizchlipala@premierhistology.com
www.premierhistology.com
- -----Original Message-----
From: Anita Westbrook [mailto:awestbr@sehosp.org]
Sent: Thursday, May 01, 2003 9:39 AM
To: histonet
Subject: Decal Solutions
Since we are on the subject of decalcification, I was wondering what
brand
of decal solutions everyone is using?
Thanks! Anita
This email and any files transmitted with it are confidential and
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If you have received this email in error please contact the system
administrator for Southeast Missouri Hospital at infosys@sehosp.org.
----------------------------------------------------------------------
Date: 1 May 2003 14:16:31 -0500
From: Shey-Cherng Tzou
Subject:
Hi
I am working on murine lung frozen sections and there's been difficulties
to
staing CD3,
CD19. Does anyone have suggestion about bocking staining, antibody dilution
etc.?
Thanks!!!
SC Tzou
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----------------------------------------------------------------------
Date: 1 May 2003 14:16:49 -0500
From: Baranowski
Subject: Items for sale
Hard Tissue Histonetters,
I have consumables for sale at good prices. I had a small lab that I
have closed and have left over Buehler and Allied brand products I would
like to sell. I have various grades of alumina, various grades of
silicon carbide grinding papers, polishing cloths, microscope slide
boxes, media bottles, 10 ml. glass pipets, formalin neutralizer, etc.
Any takers? If your interested, e-mail me and I will e-mail a list with
prices to you.
Cindy Baranowski
CLB Enterprises
Atlanta, GA
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contained in the following MIME Information.
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This is a multi-part message in MIME format.
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Message
Hard Tissue
Histonetters,
I have
consumables
for sale at good prices. I had a small lab that I have closed and have
left over Buehler and Allied brand products I would like to sell. I
have
various grades of alumina, various grades of silicon carbide grinding
papers,
polishing cloths, microscope slide boxes, media bottles, 10 ml. glass
pipets, formalin neutralizer, etc. Any takers? If your
interested,
e-mail me and I will e-mail a list with prices to you.
Cindy
Baranowski
CLB
Enterprises
Atlanta,
GA
- --Boundary_(ID_wlyJRZm8VoLHtLHXQI8xCQ)--
----------------------------------------------------------------------
Date: 1 May 2003 16:31:00 -0500
From: Rae Staskiewicz
Subject: Any position openings to announce
If you would like to have any positions posted at the Illinois Society for
Histotechnologists annual meeting, please send pertinent information to Dana
Spears at dspears@agr.state.il.us before May 13.
Rae Ann Staskiewicz
President
Illinois Society for Histotechnologists
----------------------------------------------------------------------
Date: 1 May 2003 19:45:23 -0500
From: ian clarke
Subject: Re -Biogenex Optimax Immunostainer
Hi all,
I would like to know if anyone in the UK or Ireland may be interested in
purchasing the above stainer as we have one that was only used for approx 2
weeks.If anyone is interested in this machine please contact me.
Ian
----------------------------------------------------------------------
Date: 1 May 2003 19:45:41 -0500
From: LMHAMILTON1@aol.com
Subject: Achrived tissue viability when stored in 10% formalin
Hello,
The company I work for has been asked by one of our clients how long tissue
(animal) can be stored in formalin and still be viable. We have gone back
to
tissue stored for ~10yrs. but the staining was poor. If there is any advice
on where we can find documentation of this information it would be
appreciated. Any thoughts on the time frame also are welcome.
Thank You,
Loriann
******************* NOTE *******************
There may be important message content
contained in the following MIME Information.
********************************************
- ------------------ MIME Information follows ------------------
- --Boundary_(ID_kx7Bv7ry08Lg9zme0JFRrA)
Content-type: text/plain; charset=US-ASCII
Content-transfer-encoding: 7BIT
<<<<<< See above "Message Body" >>>>>>
- --Boundary_(ID_kx7Bv7ry08Lg9zme0JFRrA)
Content-type: text/html; charset=US-ASCII
Content-transfer-encoding: 7BIT
Hello,
The company I work for has been asked by one of our clients how long
tissue (animal) can be stored in formalin and still be viable. We have
gone back to tissue stored for ~10yrs. but the staining was poor. If
there is any advice on where we can find documentation of this information
it
would be appreciated. Any thoughts on the time frame also are welcome.
Thank You,
Loriann
- --Boundary_(ID_kx7Bv7ry08Lg9zme0JFRrA)--
----------------------------------------------------------------------
Date: 1 May 2003 21:16:21 -0500
From: Rowani
Subject: Re: Re -Biogenex Optimax Immunostainer
Hi all
I am working with brain of MPS IIIA mice embryos. Where could I find
references about normal histology and ultrastructure of brain of mice
embryos (from 9.5 dpc to 19.5 dpc) ?
Regards
Rowani Mohd Rawi
PhD student
Lysosomal Diseases Research Unit
Department of Chemical Pathology
Women's and Children's Hospital
72 King William Road,
5006 North Adelaide, South Australia
----------------------------------------------------------------------
Date: 1 May 2003 21:45:52 -0500
From: Rowani
Subject: mice embryo histology
Hi all
I am working with brain of MPS IIIA mice embryos. Where could I find
references about normal histology and ultrastructure of brain of mice
embryos (from 9.5 dpc to 19.5 dpc) ?
Regards
Rowani Mohd Rawi
PhD student
Lysosomal Diseases Research Unit
Department of Chemical Pathology
Women's and Children's Hospital
72 King William Road,
5006 North Adelaide, South Australia
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